2d3b

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[[Image:2d3b.gif|left|200px]]<br /><applet load="2d3b" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2d3b, resolution 3.50&Aring;" />
 
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'''Crystal Structure of the Maize Glutamine Synthetase complexed with AMPPNP and Methionine sulfoximine'''<br />
 
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==Overview==
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==Crystal Structure of the Maize Glutamine Synthetase complexed with AMPPNP and Methionine sulfoximine==
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Plants provide nourishment for animals and other heterotrophs as the sole primary producer in the food chain. Glutamine synthetase (GS), one of the essential enzymes for plant autotrophy catalyzes the incorporation of ammonia into glutamate to generate glutamine with concomitant hydrolysis of ATP, and plays a crucial role in the assimilation and re-assimilation of ammonia derived from a wide variety of metabolic processes during plant growth and development. Elucidation of the atomic structure of higher plant GS is important to understand its detailed reaction mechanism and to obtain further insight into plant productivity and agronomical utility. Here we report the first crystal structures of maize (Zea mays L.) GS. The structure reveals a unique decameric structure that differs significantly from the bacterial GS structure. Higher plants have several isoenzymes of GS differing in heat stability and catalytic properties for efficient responses to variation in the environment and nutrition. A key residue responsible for the heat stability was found to be Ile-161 in GS1a. The three structures in complex with substrate analogues, including phosphinothricin, a widely used herbicide, lead us to propose a mechanism for the transfer of phosphate from ATP to glutamate and to interpret the inhibitory action of phosphinothricin as a guide for the development of new potential herbicides.
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<StructureSection load='2d3b' size='340' side='right'caption='[[2d3b]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2d3b]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D3B FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MSL:(2S)-2-AMINO-4-(METHYLSULFONIMIDOYL)BUTANOIC+ACID'>MSL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d3b OCA], [https://pdbe.org/2d3b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d3b RCSB], [https://www.ebi.ac.uk/pdbsum/2d3b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d3b ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLNA3_MAIZE GLNA3_MAIZE] Plays a role in the flow of nitrogen into nitrogenous organic compounds.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d3/2d3b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d3b ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2D3B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=MSL:'>MSL</scene> and <scene name='pdbligand=ANP:'>ANP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3B OCA].
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*[[Glutamine synthetase 3D structures|Glutamine synthetase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Atomic structure of plant glutamine synthetase: a key enzyme for plant productivity., Unno H, Uchida T, Sugawara H, Kurisu G, Sugiyama T, Yamaya T, Sakakibara H, Hase T, Kusunoki M, J Biol Chem. 2006 Sep 29;281(39):29287-96. Epub 2006 Jul 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16829528 16829528]
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[[Category: Large Structures]]
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[[Category: Glutamate--ammonia ligase]]
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[[Category: Single protein]]
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[[Category: Zea mays]]
[[Category: Zea mays]]
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[[Category: Hase, T.]]
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[[Category: Hase T]]
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[[Category: Kurisu, G.]]
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[[Category: Kurisu G]]
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[[Category: Kusunoki, M.]]
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[[Category: Kusunoki M]]
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[[Category: Sakakibara, H.]]
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[[Category: Sakakibara H]]
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[[Category: Sugawara, H.]]
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[[Category: Sugawara H]]
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[[Category: Sugiyama, T.]]
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[[Category: Sugiyama T]]
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[[Category: Uchida, T.]]
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[[Category: Uchida T]]
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[[Category: Unno, H.]]
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[[Category: Unno H]]
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[[Category: Yamaya, T.]]
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[[Category: Yamaya T]]
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[[Category: ANP]]
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[[Category: MN]]
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[[Category: MSL]]
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[[Category: glutamine synthetase maize]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:54:55 2008''
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Current revision

Crystal Structure of the Maize Glutamine Synthetase complexed with AMPPNP and Methionine sulfoximine

PDB ID 2d3b

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