2d3w

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[[Image:2d3w.gif|left|200px]]<br /><applet load="2d3w" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2d3w, resolution 2.5&Aring;" />
 
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'''Crystal Structure of Escherichia coli SufC, an ATPase compenent of the SUF iron-sulfur cluster assembly machinery'''<br />
 
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==Overview==
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==Crystal Structure of Escherichia coli SufC, an ATPase compenent of the SUF iron-sulfur cluster assembly machinery==
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SufC is an ATPase component of the SUF machinery, which is involved in the biosynthesis of Fe-S clusters. To gain insight into the function of this protein, we have determined the crystal structure of Escherichia coli SufC at 2.5A resolution. Despite the similarity of the overall structure with ABC-ATPases (nucleotide-binding domains of ABC transporters), some key differences were observed. Glu171, an invariant residue involved in ATP hydrolysis, is rotated away from the nucleotide-binding pocket to form a SufC-specific salt bridge with Lys152. Due to this salt bridge, D-loop that follows Glu171 is flipped out to the molecular surface, which may sterically inhibit the formation of an active dimer. Thus, the salt bridge may play a critical role in regulating ATPase activity and preventing wasteful ATP hydrolysis. Furthermore, SufC has a unique Q-loop structure on its surface, which may form a binding site for its partner proteins, SufB and/or SufD.
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<StructureSection load='2d3w' size='340' side='right'caption='[[2d3w]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2d3w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D3W FirstGlance]. <br>
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2D3W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3W OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d3w OCA], [https://pdbe.org/2d3w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d3w RCSB], [https://www.ebi.ac.uk/pdbsum/2d3w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d3w ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of Escherichia coli SufC, an ABC-type ATPase component of the SUF iron-sulfur cluster assembly machinery., Kitaoka S, Wada K, Hasegawa Y, Minami Y, Fukuyama K, Takahashi Y, FEBS Lett. 2006 Jan 9;580(1):137-43. Epub 2005 Dec 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16364320 16364320]
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== Function ==
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[https://www.uniprot.org/uniprot/SUFC_ECOLI SUFC_ECOLI] Has low ATPase activity. The SufBCD complex acts synergistically with SufE to stimulate the cysteine desulfurase activity of SufS. The SufBCD complex contributes to the assembly or repair of oxygen-labile iron-sulfur clusters under oxidative stress. May facilitate iron uptake from extracellular iron chelators under iron limitation.<ref>PMID:12554644</ref> <ref>PMID:12941942</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d3/2d3w_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d3w ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fukuyama, K.]]
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[[Category: Fukuyama K]]
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[[Category: Hasegawa, Y.]]
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[[Category: Hasegawa Y]]
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[[Category: Kitaoka, S.]]
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[[Category: Kitaoka S]]
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[[Category: Minami, Y.]]
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[[Category: Minami Y]]
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[[Category: Takahashi, Y.]]
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[[Category: Takahashi Y]]
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[[Category: Wada, K.]]
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[[Category: Wada K]]
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[[Category: abc-atpase]]
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[[Category: abc-transporter]]
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[[Category: crystal structure]]
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[[Category: iron-sulfur cluster]]
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[[Category: sufc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:55:07 2008''
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Current revision

Crystal Structure of Escherichia coli SufC, an ATPase compenent of the SUF iron-sulfur cluster assembly machinery

PDB ID 2d3w

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