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| - | [[Image:2dfz.jpg|left|200px]]<br /><applet load="2dfz" size="350" color="white" frame="true" align="right" spinBox="true" | + | #REDIRECT [[2zyk]] This PDB entry is obsolete and replaced by 2zyk |
| - | caption="2dfz, resolution 2.50Å" />
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| - | '''Crystal structure of cyclodextrin-binding protein complexed with gamma-cyclodextrin'''<br />
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| - | ==Overview==
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| - | The crystal structure of a Thermoactinomyces vulgaris cyclo/maltodextrin-binding protein (TvuCMBP) complexed with gamma-cyclodextrin has been determined. Like Escherichia coli maltodextrin-binding protein (EcoMBP) and other bacterial sugar-binding proteins, TvuCMBP consists of two domains, an N- and a C-domain, both of which are composed of a central beta-sheet surrounded by alpha-helices; the domains are joined by a hinge region containing three segments. gamma-Cyclodextrin is located at a cleft formed by the two domains. A common functional conformational change has been reported in this protein family, which involves switching from an open form to a sugar-transporter bindable form, designated a closed form. The TvuCMBP-gamma-cyclodextrin complex structurally resembles the closed form of EcoMBP, indicating that TvuCMBP complexed with gamma-cyclodextrin adopts the closed form. The fluorescence measurements also showed that the affinities of TvuCMBP for cyclodextrins were almost equal to those for maltooligosaccharides. Despite having similar folds, the sugar-binding site of the N-domain part of TvuCMBP and other bacterial sugar-binding proteins are strikingly different. In TvuCMBP, the side-chain of Leu59 protrudes from the N-domain part into the sugar-binding cleft and orients toward the central cavity of gamma-cyclodextrin, thus Leu59 appears to play the key role in binding. The cleft of the sugar-binding site of TvuCMBP is also wider than that of EcoMBP. These findings suggest that the sugar-binding site of the N-domain part and the wide cleft are critical in determining the specificity of TvuCMBP for gamma-cyclodextrin.
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| - | ==About this Structure==
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| - | 2DFZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoactinomyces_vulgaris Thermoactinomyces vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DFZ OCA].
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| - | ==Reference==
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| - | Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo/maltodextrin-binding protein., Tonozuka T, Sogawa A, Yamada M, Matsumoto N, Yoshida H, Kamitori S, Ichikawa K, Mizuno M, Nishikawa A, Sakano Y, FEBS J. 2007 Apr;274(8):2109-20. Epub 2007 Mar 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17371546 17371546]
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| - | [[Category: Single protein]]
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| - | [[Category: Thermoactinomyces vulgaris]]
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| - | [[Category: Ichikawa, K.]]
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| - | [[Category: Kamitori, S.]]
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| - | [[Category: Matsumoto, N.]]
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| - | [[Category: Mizuno, M.]]
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| - | [[Category: Nishikawa, A.]]
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| - | [[Category: Sakano, Y.]]
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| - | [[Category: Sogawa, A.]]
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| - | [[Category: Tonozuka, T.]]
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| - | [[Category: Yamada, M.]]
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| - | [[Category: Yoshida, H.]]
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| - | [[Category: cyclodextrin]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:58:23 2008''
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