1j5l

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:28, 16 August 2023) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
{{STRUCTURE_1j5l| PDB=1j5l | SCENE= }}
 
-
===NMR STRUCTURE OF THE ISOLATED BETA_C DOMAIN OF LOBSTER METALLOTHIONEIN-1===
 
-
{{ABSTRACT_PUBMED_12203008}}
 
-
==Function==
+
==NMR STRUCTURE OF THE ISOLATED BETA_C DOMAIN OF LOBSTER METALLOTHIONEIN-1==
-
[[http://www.uniprot.org/uniprot/MT1_HOMAM MT1_HOMAM]] Metallothioneins have a high content of cysteine residues that bind various heavy metals. The different forms of lobster metallothioneins may have different biological functions. Class I MTS in marine crustacea are involved in the sequestration of elevated levels of heavy-metal ions. Binds 6 metal ions. Known to bind cadmium.
+
<StructureSection load='1j5l' size='340' side='right'caption='[[1j5l]]' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1j5l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homarus_americanus Homarus americanus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1hzq 1hzq]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J5L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J5L FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j5l OCA], [https://pdbe.org/1j5l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j5l RCSB], [https://www.ebi.ac.uk/pdbsum/1j5l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j5l ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/MT1_HOMAM MT1_HOMAM] Metallothioneins have a high content of cysteine residues that bind various heavy metals. The different forms of lobster metallothioneins may have different biological functions. Class I MTS in marine crustacea are involved in the sequestration of elevated levels of heavy-metal ions. Binds 6 metal ions. Known to bind cadmium.
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The three-dimensional structures of the isolated Cd(3)beta domains from Homarus americanus metallothionein have been determined by NMR methods in order to establish a set of beta-domain structures for comparative analysis. First, it was determined that the Cd-cysteine connectivities forming the Cd(3)S(9) metal center were identical to those observed for the beta(N) domain in the native holoprotein. Time- and temperature-dependence studies of the (113)Cd and (1)H 1D-NMR spectra indicated that the beta(N) domain undergoes slow conformational changes before reaching an equilibrium structure. In addition to structural information provided by the metal-to-cysteine connectivities, Phi, chi(1) and chi(2) angle constraints, three H(N...)S hydrogen bond interactions were also determined from a long-range optimized (1)H(N)-(113)Cd HMQC experiment. A simulated annealing protocol was applied to the distance and angle constraints obtained from the 2D-NMR experiments to calculate the three-dimensional structure of the synthetic Cd(3)beta(N) domain of lobster metallothionein. Structure-reactivity relationships are proposed for the reactions of Cd(3)beta domains with 5,5'-dithiobis(2-nitrobenzoate), based on comparisons of surface exposure of sulfur atoms of the lobster and rabbit Cd(3)beta domain structures. Finally, the surface exposure of the beta domains of lobster is compared with beta domains from mammalian metallothioneins.
-
==About this Structure==
+
Structure of the (113)Cd(3)beta domains from Homarus americanus metallothionein-1: hydrogen bonding and solvent accessibility of sulfur atoms.,Munoz A, Forsterling FH, Shaw CF 3rd, Petering DH J Biol Inorg Chem. 2002 Sep;7(7-8):713-24. Epub 2002 Mar 13. PMID:12203008<ref>PMID:12203008</ref>
-
[[1j5l]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1hzq 1hzq]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J5L OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<ref group="xtra">PMID:012203008</ref><references group="xtra"/><references/>
+
</div>
-
[[Category: Forsterling, F H.]]
+
<div class="pdbe-citations 1j5l" style="background-color:#fffaf0;"></div>
-
[[Category: III, C F.Shaw.]]
+
== References ==
-
[[Category: Munoz, A.]]
+
<references/>
-
[[Category: Petering, D H.]]
+
__TOC__
-
[[Category: Beta-domain]]
+
</StructureSection>
-
[[Category: Cadmium-sulfur-cluster]]
+
[[Category: Homarus americanus]]
-
[[Category: Metal binding protein]]
+
[[Category: Large Structures]]
-
[[Category: Metalloprotein]]
+
[[Category: Forsterling FH]]
 +
[[Category: Munoz A]]
 +
[[Category: Petering DH]]
 +
[[Category: Shaw III CF]]

Current revision

NMR STRUCTURE OF THE ISOLATED BETA_C DOMAIN OF LOBSTER METALLOTHIONEIN-1

PDB ID 1j5l

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1j5l"
Personal tools