3irp

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{{STRUCTURE_3irp| PDB=3irp | SCENE= }}
 
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===Crystal structure of functional region of UafA from Staphylococcus saprophyticus at 1.50 angstrom resolution===
 
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{{ABSTRACT_PUBMED_21162026}}
 
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==About this Structure==
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==Crystal structure of functional region of UafA from Staphylococcus saprophyticus at 1.50 angstrom resolution==
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[[3irp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_saprophyticus_subsp._saprophyticus Staphylococcus saprophyticus subsp. saprophyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IRP OCA].
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<StructureSection load='3irp' size='340' side='right'caption='[[3irp]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3irp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_saprophyticus_subsp._saprophyticus_ATCC_15305_=_NCTC_7292 Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IRP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IRP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3irp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3irp OCA], [https://pdbe.org/3irp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3irp RCSB], [https://www.ebi.ac.uk/pdbsum/3irp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3irp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UAFA_STAS1 UAFA_STAS1]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ir/3irp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3irp ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Staphylococci use cell wall-anchored proteins as adhesins to attach to host tissues. Staphylococcus saprophyticus, a uropathogenic species, has a unique cell wall-anchored protein, uro-adherence factor A (UafA), which shows erythrocyte binding activity. To investigate the mechanism of adhesion by UafA, we determined the crystal structure of the functional region of UafA at 1.5 A resolution. The structure was composed of three domains, designated as the N2, N3, and B domains, arranged in a triangular relative configuration. Hemagglutination inhibition assay with domain-truncated mutants indicated that both N and B domains were necessary for erythrocyte binding. Based on these results, a novel manner of ligand binding in which the B domain acts as a functional domain was proposed as the adhesion mechanism of S. saprophyticus.
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==Reference==
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Crystal structure of the functional region of Uro-adherence factor A from Staphylococcus saprophyticus reveals participation of the B domain in ligand binding.,Matsuoka E, Tanaka Y, Kuroda M, Shouji Y, Ohta T, Tanaka I, Yao M Protein Sci. 2011 Feb;20(2):406-16. doi: 10.1002/pro.573. PMID:21280131<ref>PMID:21280131</ref>
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<ref group="xtra">PMID:021162026</ref><references group="xtra"/><references/>
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[[Category: Staphylococcus saprophyticus subsp. saprophyticus]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Kuroda, M.]]
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</div>
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[[Category: Matsuoka, E.]]
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<div class="pdbe-citations 3irp" style="background-color:#fffaf0;"></div>
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[[Category: Shouji, Y.]]
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== References ==
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[[Category: Tanaka, I.]]
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<references/>
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[[Category: Tanaka, Y.]]
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__TOC__
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[[Category: Yao, M.]]
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</StructureSection>
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[[Category: Cell adhesion]]
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[[Category: Large Structures]]
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[[Category: Cell wall]]
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[[Category: Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292]]
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[[Category: Dev-igg fold]]
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[[Category: Kuroda M]]
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[[Category: Hemagglutinin]]
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[[Category: Matsuoka E]]
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[[Category: Peptidoglycan-anchor]]
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[[Category: Shouji Y]]
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[[Category: Secreted]]
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[[Category: Tanaka I]]
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[[Category: Virulence]]
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[[Category: Tanaka Y]]
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[[Category: Yao M]]

Current revision

Crystal structure of functional region of UafA from Staphylococcus saprophyticus at 1.50 angstrom resolution

PDB ID 3irp

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