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3jsc

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{{STRUCTURE_3jsc| PDB=3jsc | SCENE= }}
 
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===CcdBVfi-FormI-pH7.0===
 
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{{ABSTRACT_PUBMED_19959472}}
 
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==About this Structure==
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==CcdBVfi-FormI-pH7.0==
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[[3jsc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aliivibrio_fischeri Aliivibrio fischeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JSC OCA].
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<StructureSection load='3jsc' size='340' side='right'caption='[[3jsc]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3jsc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aliivibrio_fischeri Aliivibrio fischeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JSC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JSC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jsc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jsc OCA], [https://pdbe.org/3jsc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jsc RCSB], [https://www.ebi.ac.uk/pdbsum/3jsc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jsc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q84B82_ALIFS Q84B82_ALIFS]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/js/3jsc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3jsc ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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CcdB(Vfi) from Vibrio fischeri is a member of the CcdB family of toxins that poison covalent gyrase-DNA complexes. In solution CcdB(Vfi) is a dimer that unfolds to the corresponding monomeric components in a two-state fashion. In the unfolded state, the monomer retains a partial secondary structure. This observation correlates well with the crystal and NMR structures of the protein, which show a dimer with a hydrophobic core crossing the dimer interface. In contrast to its F plasmid homologue, CcdB(Vfi) possesses a rigid dimer interface, and the apparent relative rotations of the two subunits are due to structural plasticity of the monomer. CcdB(Vfi) shows a number of non-conservative substitutions compared with the F plasmid protein in both the CcdA and the gyrase binding sites. Although variation in the CcdA interaction site likely determines toxin-antitoxin specificity, substitutions in the gyrase-interacting region may have more profound functional implications.
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==Reference==
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Structural and thermodynamic characterization of Vibrio fischeri CcdB.,De Jonge N, Hohlweg W, Garcia-Pino A, Respondek M, Buts L, Haesaerts S, Lah J, Zangger K, Loris R J Biol Chem. 2010 Feb 19;285(8):5606-13. Epub 2009 Dec 2. PMID:19959472<ref>PMID:19959472</ref>
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<ref group="xtra">PMID:019959472</ref><ref group="xtra">PMID:017401216</ref><references group="xtra"/><references/>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3jsc" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Aliivibrio fischeri]]
[[Category: Aliivibrio fischeri]]
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[[Category: Buts, L.]]
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[[Category: Large Structures]]
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[[Category: Jonge, N De.]]
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[[Category: Buts L]]
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[[Category: Loris, R.]]
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[[Category: De Jonge N]]
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[[Category: Alpha+beta]]
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[[Category: Loris R]]
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[[Category: Sh3 domain]]
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[[Category: Toxin]]
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Current revision

CcdBVfi-FormI-pH7.0

PDB ID 3jsc

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