3jtq

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{{STRUCTURE_3jtq| PDB=3jtq | SCENE= }}
 
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===Mutations in Cephalosporin Acylase Affecting Stability and Autoproteolysis===
 
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{{ABSTRACT_PUBMED_19800869}}
 
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==About this Structure==
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==Mutations in Cephalosporin Acylase Affecting Stability and Autoproteolysis==
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[[3jtq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JTQ OCA].
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<StructureSection load='3jtq' size='340' side='right'caption='[[3jtq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3jtq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._GK16 Pseudomonas sp. GK16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JTQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JTQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jtq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jtq OCA], [https://pdbe.org/3jtq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jtq RCSB], [https://www.ebi.ac.uk/pdbsum/3jtq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jtq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A4ZVL3_PSEU7 A4ZVL3_PSEU7]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/3jtq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3jtq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cephalosporin acylase (CA), a member of the N-terminal nucleophile hydrolase family, is activated through two steps of intramolecular autoproteolysis, the first mediated by a serine residue, and the second by a glutamate, which releases the pro-segment and produces an active enzyme. In this study, we have determined the crystal structures of mutants which could affect primary or secondary auto-cleavage and of sequential intermediates of a slow-processing mutant at 2.0-2.5A resolutions. The pro-segments of the mutants undergo dynamic conformational changes during activation and adopt surprisingly different loop conformations from one another. However, the autoproteolytic site was found to form a catalytically competent conformation with a solvent water molecule, which was essentially conserved in the CA mutants.
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==Reference==
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Structural features of cephalosporin acylase reveal the basis of autocatalytic activation.,Cho KJ, Kim JK, Lee JH, Shin HJ, Park SS, Kim KH Biochem Biophys Res Commun. 2009 Dec 11;390(2):342-8. Epub 2009 Oct 2. PMID:19800869<ref>PMID:19800869</ref>
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<ref group="xtra">PMID:019800869</ref><references group="xtra"/><references/>
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[[Category: Glutaryl-7-aminocephalosporanic-acid acylase]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Pseudomonas sp.]]
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</div>
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[[Category: Cho, K J.]]
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<div class="pdbe-citations 3jtq" style="background-color:#fffaf0;"></div>
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[[Category: Kim, J K.]]
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[[Category: Kim, K H.]]
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==See Also==
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[[Category: Lee, J H.]]
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*[[Cephalosporin acylase 3D structures|Cephalosporin acylase 3D structures]]
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[[Category: Park, S S.]]
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== References ==
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[[Category: Shin, H J.]]
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<references/>
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[[Category: Autoproteolysis]]
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__TOC__
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[[Category: Cephalosporin acylase]]
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</StructureSection>
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[[Category: Hydrolase]]
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[[Category: Large Structures]]
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[[Category: Pseudomonas sp. GK16]]
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[[Category: Cho KJ]]
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[[Category: Kim JK]]
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[[Category: Kim KH]]
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[[Category: Lee JH]]
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[[Category: Park SS]]
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[[Category: Shin HJ]]

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Mutations in Cephalosporin Acylase Affecting Stability and Autoproteolysis

PDB ID 3jtq

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