|
|
| (3 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:2ds3.gif|left|200px]]<br /><applet load="2ds3" size="350" color="white" frame="true" align="right" spinBox="true" | + | #REDIRECT [[3d3i]] This PDB entry is obsolete and replaced by 3d3i |
| - | caption="2ds3, resolution 1.78Å" />
| + | |
| - | '''Crystal structure of E. coli YgjK, a member of glycosyl hydrolase family 63'''<br />
| + | |
| - | | + | |
| - | ==Overview==
| + | |
| - | Processing alpha-glucosidase I, which is classified into glycosyl hydrolase (GH) family 63, hydrolyzes an oligosaccharide precursor of eukaryotic N-linked glycoproteins. Recently, many bacteria have been reported to possess genes for proteins that are homologous to the GH family 63 glucosidases. In this paper, Escherichia coli K12 YgjK protein, a member of GH family 63, was overexpressed, purified and crystallized using the vapour-diffusion method. Diffraction data were collected to 1.8 A resolution and the crystal was found to belong to the monoclinic space group P2(1), with unit-cell parameters a = 88.5, b = 137.1, c = 60.9 A, beta = 98.1 degrees. The V(M) value was determined to be 2.1 A(3) Da(-1), which corresponds to the presence of two protein molecules in the asymmetric unit.
| + | |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 2DS3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DS3 OCA].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | Crystallization and preliminary X-ray analysis of Escherichia coli K12 YgjK protein, a member of glycosyl hydrolase family 63., Tonozuka T, Uechi A, Mizuno M, Ichikawa K, Nishikawa A, Sakano Y, Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1284-5. Epub 2004, Jun 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15213393 15213393]
| + | |
| - | [[Category: Escherichia coli]]
| + | |
| - | [[Category: Single protein]]
| + | |
| - | [[Category: Kurakata, Y.]]
| + | |
| - | [[Category: Tonozuka, T.]]
| + | |
| - | [[Category: CA]]
| + | |
| - | [[Category: GOL]]
| + | |
| - | [[Category: escherichia coli]]
| + | |
| - | [[Category: glycosyl hydrolase family 63]]
| + | |
| - | [[Category: processing alpha-glucosidase]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:02:01 2008''
| + | |
