3k8p

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{{STRUCTURE_3k8p| PDB=3k8p | SCENE= }}
 
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===Structural basis for vesicle tethering by the Dsl1 complex===
 
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{{ABSTRACT_PUBMED_20005805}}
 
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==Function==
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==Structural basis for vesicle tethering by the Dsl1 complex==
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[[http://www.uniprot.org/uniprot/SEC39_YEAST SEC39_YEAST]] Required for protein transport between the Golgi and the endoplasmic reticulum. May contribute to tethering of coatomer-coated retrograde transport vesicles to the ER membrane through interaction with and stabilization of the SNARE complex.<ref>PMID:15242642</ref> <ref>PMID:15958492</ref>
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<StructureSection load='3k8p' size='340' side='right'caption='[[3k8p]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3k8p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K8P FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k8p OCA], [https://pdbe.org/3k8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k8p RCSB], [https://www.ebi.ac.uk/pdbsum/3k8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k8p ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6CUS2_KLULA Q6CUS2_KLULA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k8/3k8p_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3k8p ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Vesicle trafficking requires membrane fusion, mediated by SNARE proteins, and upstream events that probably include "tethering," an initial long-range attachment between a vesicle and its target organelle. Among the factors proposed to mediate tethering are a set of multisubunit tethering complexes (MTCs). The Dsl1 complex, with only three subunits, is the simplest known MTC and is essential for the retrograde traffic of COPI-coated vesicles from the Golgi to the ER. To elucidate structural principles underlying MTC function, we have determined the structure of the Dsl1 complex, revealing a tower containing at its base the binding sites for two ER SNAREs and at its tip a flexible lasso for capturing vesicles. The Dsl1 complex binds to individual SNAREs via their N-terminal regulatory domains and also to assembled SNARE complexes; moreover, it is capable of accelerating SNARE complex assembly. Our results suggest that even the simplest MTC may be capable of orchestrating vesicle capture, uncoating, and fusion.
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==About this Structure==
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A structure-based mechanism for vesicle capture by the multisubunit tethering complex Dsl1.,Ren Y, Yip CK, Tripathi A, Huie D, Jeffrey PD, Walz T, Hughson FM Cell. 2009 Dec 11;139(6):1119-29. PMID:20005805<ref>PMID:20005805</ref>
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[[3k8p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K8P OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:020005805</ref><references group="xtra"/><references/>
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</div>
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<div class="pdbe-citations 3k8p" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Kluyveromyces lactis]]
[[Category: Kluyveromyces lactis]]
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Hughson, F M.]]
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[[Category: Hughson FM]]
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[[Category: Jeffrey, P D.]]
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[[Category: Jeffrey PD]]
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[[Category: Ren, Y.]]
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[[Category: Ren Y]]
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[[Category: Dsl1 complex]]
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[[Category: Endoplasmic reticulum]]
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[[Category: Er-golgi transport]]
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[[Category: Intracellular trafficking]]
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[[Category: Membrane]]
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[[Category: Multisubunit tethering complex]]
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[[Category: Protein transport]]
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[[Category: Snare protein]]
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[[Category: Transport]]
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[[Category: Transport protein-transport protein complex]]
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Current revision

Structural basis for vesicle tethering by the Dsl1 complex

PDB ID 3k8p

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