2bky

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{{STRUCTURE_2bky| PDB=2bky | SCENE= }}
 
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===CRYSTAL STRUCTURE OF THE ALBA1:ALBA2 HETERODIMER FROM SULFOLOBUS SOLFATARICUS===
 
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{{ABSTRACT_PUBMED_16004869}}
 
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==Function==
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==Crystal structure of the Alba1:Alba2 heterodimer from sulfolobus solfataricus==
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[[http://www.uniprot.org/uniprot/ALBA1_SULSO ALBA1_SULSO]] Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. May be involved in DNA compaction. May bind rRNA and mRNA, playing a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes.[HAMAP-Rule:MF_01122] [[http://www.uniprot.org/uniprot/ALBA2_SULSO ALBA2_SULSO]] Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes (By similarity).
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<StructureSection load='2bky' size='340' side='right'caption='[[2bky]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2bky]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BKY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bky OCA], [https://pdbe.org/2bky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bky RCSB], [https://www.ebi.ac.uk/pdbsum/2bky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bky ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ALBA1_SACS2 ALBA1_SACS2] Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. May be involved in DNA compaction. May bind rRNA and mRNA, playing a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/2bky_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bky ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Organisms growing at elevated temperatures face a particular challenge to maintain the integrity of their genetic material. All thermophilic and hyperthermophilic archaea encode one or more copies of the Alba (Sac10b) gene. Alba is an abundant, dimeric, highly basic protein that binds cooperatively and at high density to DNA. Sulfolobus solfataricus encodes a second copy of the Alba gene, and the Alba2 protein is expressed at approximately 5% of the level of Alba1. We demonstrate by NMR, ITC, and crystallography that Alba2 exists exclusively as a heterodimer with Alba1 at physiological concentrations and that heterodimerization exerts a clear effect upon the DNA packaging, as observed by EM, potentially by changing the interface between adjacent Alba dimers in DNA complexes. A functional role for Alba2 in modulation of higher order chromatin structure and DNA condensation is suggested.
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==About this Structure==
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Obligate heterodimerization of the archaeal Alba2 protein with Alba1 provides a mechanism for control of DNA packaging.,Jelinska C, Conroy MJ, Craven CJ, Hounslow AM, Bullough PA, Waltho JP, Taylor GL, White MF Structure. 2005 Jul;13(7):963-71. PMID:16004869<ref>PMID:16004869</ref>
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[[2bky]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BKY OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:016004869</ref><references group="xtra"/><references/>
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</div>
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[[Category: Sulfolobus solfataricus]]
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<div class="pdbe-citations 2bky" style="background-color:#fffaf0;"></div>
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[[Category: Bullough, P A.]]
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== References ==
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[[Category: Conroy, M J.]]
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<references/>
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[[Category: Craven, C J.]]
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__TOC__
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[[Category: Jelinska, C.]]
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</StructureSection>
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[[Category: Taylor, G L.]]
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[[Category: Large Structures]]
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[[Category: Waltho, J P.]]
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[[Category: Saccharolobus solfataricus P2]]
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[[Category: White, M F.]]
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[[Category: Bullough PA]]
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[[Category: Archaeal dna binding protein]]
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[[Category: Conroy MJ]]
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[[Category: Dna binding protein]]
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[[Category: Craven CJ]]
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[[Category: Dna condensation]]
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[[Category: Jelinska C]]
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[[Category: Dna-binding]]
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[[Category: Taylor GL]]
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[[Category: Rna-binding]]
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[[Category: Waltho JP]]
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[[Category: White MF]]

Current revision

Crystal structure of the Alba1:Alba2 heterodimer from sulfolobus solfataricus

PDB ID 2bky

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