2dx5

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[[Image:2dx5.gif|left|200px]]<br /><applet load="2dx5" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2dx5, resolution 3.35&Aring;" />
 
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'''The complex structure between the mouse EAP45-GLUE domain and ubiquitin'''<br />
 
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==Overview==
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==The complex structure between the mouse EAP45-GLUE domain and ubiquitin==
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ESCRT-II, a complex that sorts ubiquitinated membrane proteins to lysosomes, localizes to endosomes through interaction between the Vps36 subunit's GLUE domain and phosphatidylinositides (PIs). In yeast, a ubiquitin (Ub)-interacting NZF domain is inserted in Vps36 GLUE, whereas its mammalian counterpart, Eap45 GLUE, lacks the NZF domain. In the Eap45 GLUE-Ub complex structure, Ub binds far from the proposed PI-binding site of Eap45 GLUE, suggesting their independent binding.
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<StructureSection load='2dx5' size='340' side='right'caption='[[2dx5]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2dx5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DX5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.35&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dx5 OCA], [https://pdbe.org/2dx5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dx5 RCSB], [https://www.ebi.ac.uk/pdbsum/2dx5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dx5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VPS36_MOUSE VPS36_MOUSE] Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3 (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dx/2dx5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2dx5 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2DX5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DX5 OCA].
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*[[3D structures of ubiquitin|3D structures of ubiquitin]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural basis of ubiquitin recognition by mammalian Eap45 GLUE domain., Hirano S, Suzuki N, Slagsvold T, Kawasaki M, Trambaiolo D, Kato R, Stenmark H, Wakatsuki S, Nat Struct Mol Biol. 2006 Nov;13(11):1031-2. Epub 2006 Oct 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17057714 17057714]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Hirano S]]
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[[Category: Hirano, S.]]
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[[Category: Kato R]]
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[[Category: Kato, R.]]
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[[Category: Kawasaki M]]
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[[Category: Kawasaki, M.]]
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[[Category: Slagsvold T]]
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[[Category: Slagsvold, T.]]
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[[Category: Stenmark H]]
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[[Category: Stenmark, H.]]
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[[Category: Suzuki N]]
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[[Category: Suzuki, N.]]
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[[Category: Trambaiolo D]]
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[[Category: Trambaiolo, D.]]
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[[Category: Wakatsuki S]]
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[[Category: Wakatsuki, S.]]
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[[Category: protein-protein complex]]
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[[Category: ubiquitin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:03:39 2008''
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Current revision

The complex structure between the mouse EAP45-GLUE domain and ubiquitin

PDB ID 2dx5

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