3k4q

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{{STRUCTURE_3k4q| PDB=3k4q | SCENE= }}
 
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===Aspergillus niger Phytase in complex with myo-inositol hexakis sulfate===
 
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{{ABSTRACT_PUBMED_20541524}}
 
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==Function==
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==Aspergillus niger Phytase in complex with myo-inositol hexakis sulfate==
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[[http://www.uniprot.org/uniprot/PHYA_ASPNG PHYA_ASPNG]] Catalyzes the hydrolysis of inorganic orthophosphate from phytate.
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<StructureSection load='3k4q' size='340' side='right'caption='[[3k4q]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3k4q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K4Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K4Q FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IHS:D-MYO-INOSITOL-HEXASULPHATE'>IHS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k4q OCA], [https://pdbe.org/3k4q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k4q RCSB], [https://www.ebi.ac.uk/pdbsum/3k4q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k4q ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHYA_ASPNG PHYA_ASPNG] Catalyzes the hydrolysis of inorganic orthophosphate from phytate.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k4/3k4q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3k4q ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phytases hydrolyse the phosphomonoesters of phytate (myo-inositol-1,2,3,4,5,6-hexakis phosphate) and thus find uses in plant and animal production through the mobilisation of phosphorus from this source. The structure of partially deglycosylated Aspergillus niger PhyA is presented in apo form and in complex with the potent inhibitor myo-inositol-1,2,3,4,5,6-hexakis sulfate, which by analogy with phytate provides a snapshot of the Michaelis complex. The structure explains the enzyme's preference for the 3'-phosphate of phytate. The apo-and inhibitor-bound forms are similar and no induced-fit mechanism operates. Furthermore the enzyme structure is apparently unaffected by the presence of glycosides on the surface. The new structures of A. niger PhyA are discussed in the context of protein engineering studies aimed at modulating pH preference and stability.
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==About this Structure==
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The structure of Aspergillus niger phytase PhyA in complex with a phytate mimetic.,Oakley AJ Biochem Biophys Res Commun. 2010 Jul 9;397(4):745-9. Epub 2010 Jun 10. PMID:20541524<ref>PMID:20541524</ref>
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[[3k4q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K4Q OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:020541524</ref><references group="xtra"/><references/>
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</div>
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[[Category: 3-phytase]]
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<div class="pdbe-citations 3k4q" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Phytase 3D structures|Phytase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Aspergillus niger]]
[[Category: Aspergillus niger]]
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[[Category: Oakley, A J.]]
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[[Category: Large Structures]]
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[[Category: 3-phosphotase]]
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[[Category: Oakley AJ]]
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[[Category: 37288-11-2]]
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[[Category: 70701-62-1]]
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[[Category: Disulfide bond]]
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[[Category: Glycoprotein]]
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[[Category: Hydrolase]]
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[[Category: Myo-inositol hexakis phosphate phosphohydrolase]]
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[[Category: Myo-inositol hexakis sulfate]]
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[[Category: Phya]]
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[[Category: Phytase]]
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[[Category: Secreted]]
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Current revision

Aspergillus niger Phytase in complex with myo-inositol hexakis sulfate

PDB ID 3k4q

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