3lku

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "3lku" [edit=sysop:move=sysop])
Current revision (09:23, 30 October 2024) (edit) (undo)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
-
{{STRUCTURE_3lku| PDB=3lku | SCENE= }}
 
-
===Crystal structure of S. cerevisiae Get4 in complex with an N-terminal fragment of Get5===
 
-
{{ABSTRACT_PUBMED_20554915}}
 
-
==Function==
+
==Crystal structure of S. cerevisiae Get4 in complex with an N-terminal fragment of Get5==
-
[[http://www.uniprot.org/uniprot/MDY2_YEAST MDY2_YEAST]] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.<ref>PMID:10514570</ref> <ref>PMID:16390866</ref>
+
<StructureSection load='3lku' size='340' side='right'caption='[[3lku]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3lku]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LKU FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PRO:PROLINE'>PRO</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lku OCA], [https://pdbe.org/3lku PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lku RCSB], [https://www.ebi.ac.uk/pdbsum/3lku PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lku ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/GET4_YEAST GET4_YEAST] May play a role in insertion of tail-anchored proteins into the endoplasmic reticulum membrane.<ref>PMID:19325107</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lk/3lku_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lku ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The recently elucidated Get proteins are responsible for the targeted delivery of the majority of tail-anchored (TA) proteins to the endoplasmic reticulum. Get4 and Get5 have been identified in the early steps of the pathway mediating TA substrate delivery to the cytoplasmic targeting factor Get3. Here we report a crystal structure of Get4 and an N-terminal fragment of Get5 from Saccharomyces cerevisae. We show Get4 and Get5 (Get4/5) form an intimate complex that exists as a dimer (two copies of Get4/5) mediated by the C-terminus of Get5. We further demonstrate that Get3 specifically binds to a conserved surface on Get4 in a nucleotide dependent manner. This work provides further evidence for a model in which Get4/5 operates upstream of Get3 and mediates the specific delivery of a TA substrate.
-
==About this Structure==
+
Structural characterization of the Get4/Get5 complex and its interaction with Get3.,Chartron JW, Suloway CJ, Zaslaver M, Clemons WM Jr Proc Natl Acad Sci U S A. 2010 Jul 6;107(27):12127-32. Epub 2010 Jun 16. PMID:20554915<ref>PMID:20554915</ref>
-
[[3lku]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKU OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<ref group="xtra">PMID:020554915</ref><references group="xtra"/><references/>
+
</div>
 +
<div class="pdbe-citations 3lku" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Chartron, J W.]]
+
[[Category: Chartron JW]]
-
[[Category: Clemons, W M.]]
+
[[Category: Clemons Jr WM]]
-
[[Category: Alpha helical repeat]]
+
-
[[Category: Protein binding]]
+
-
[[Category: Tpr-like]]
+

Current revision

Crystal structure of S. cerevisiae Get4 in complex with an N-terminal fragment of Get5

PDB ID 3lku

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3lku"
Personal tools