3l7k

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{{STRUCTURE_3l7k| PDB=3l7k | SCENE= }}
 
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===Structure of the Wall Teichoic Acid Polymerase TagF, H444N + CDPG (15 minute soak)===
 
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{{ABSTRACT_PUBMED_20400947}}
 
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==About this Structure==
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==Structure of the Wall Teichoic Acid Polymerase TagF, H444N + CDPG (15 minute soak)==
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[[3l7k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L7K OCA].
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<StructureSection load='3l7k' size='340' side='right'caption='[[3l7k]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3l7k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_epidermidis_RP62A Staphylococcus epidermidis RP62A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L7K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L7K FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C2G:[CYTIDINE-5-PHOSPHATE]+GLYCERYLPHOSPHORIC+ACID+ESTER'>C2G</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EDT:{[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC+ACID'>EDT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l7k OCA], [https://pdbe.org/3l7k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l7k RCSB], [https://www.ebi.ac.uk/pdbsum/3l7k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l7k ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TAGF_STAEQ TAGF_STAEQ] Responsible for the polymerization of the main chain of the major teichoic acid by sequential transfer of glycerol phosphate units from CDP-glycerol to the disaccharide linkage unit. Synthesizes polymers of approximately 35 glycerol phosphate units in length.<ref>PMID:20400947</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l7/3l7k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3l7k ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Teichoic acid polymers are composed of polyol-phosphate units and form a major component of Gram-positive bacterial cell walls. These anionic compounds perform a multitude of important roles in bacteria and are synthesized by monotopic membrane proteins of the TagF polymerase family. We have determined the structure of Staphylococcus epidermidis TagF to 2.7-A resolution from a construct that includes both the membrane-targeting region and the glycerol-phosphate polymerase domains. TagF possesses a helical region for interaction with the lipid bilayer, placing the active site at a suitable distance for access to the membrane-bound substrate. Characterization of active-site residue variants and analysis of a CDP-glycerol substrate complex suggest a mechanism for polymer synthesis. With the importance of teichoic acid in Gram-positive physiology, this elucidation of the molecular details of TagF function provides a critical new target in the development of novel anti-infectives.
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==Reference==
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Structure of the bacterial teichoic acid polymerase TagF provides insights into membrane association and catalysis.,Lovering AL, Lin LY, Sewell EW, Spreter T, Brown ED, Strynadka NC Nat Struct Mol Biol. 2010 May;17(5):582-9. Epub 2010 Apr 18. PMID:20400947<ref>PMID:20400947</ref>
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<ref group="xtra">PMID:020400947</ref><references group="xtra"/><references/>
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[[Category: Staphylococcus epidermidis]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Lovering, A L.]]
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</div>
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[[Category: Strynadka, N C.J.]]
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<div class="pdbe-citations 3l7k" style="background-color:#fffaf0;"></div>
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[[Category: Gt-b fold]]
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== References ==
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[[Category: Monotopic membrane protein]]
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<references/>
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[[Category: Structural protein]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Staphylococcus epidermidis RP62A]]
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[[Category: Lovering AL]]
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[[Category: Strynadka NCJ]]

Current revision

Structure of the Wall Teichoic Acid Polymerase TagF, H444N + CDPG (15 minute soak)

PDB ID 3l7k

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