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1ovw

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[[Image:1ovw.gif|left|200px]]<br />
 
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<applet load="1ovw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ovw, resolution 2.7&Aring;" />
 
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'''ENDOGLUCANASE I COMPLEXED WITH NON-HYDROLYSABLE SUBSTRATE ANALOGUE'''<br />
 
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==Overview==
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==ENDOGLUCANASE I COMPLEXED WITH NON-HYDROLYSABLE SUBSTRATE ANALOGUE==
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Endoglucanase I (EG I) is a cellulase, from glycosyl hydrolase family 7, which cleaves the beta-1,4 linkages of cellulose with overall retention of, configuration. The structure of the EG I from Fusarium oxysproum, complexed to a nonhydrolyzable thiooligosaccharide substrate analogue, has, been determined by X-ray crystallography at a resolution of 2.7 A, utilizing the 4-fold noncrystallographic symmetry present in the, asymmetric unit. The electron density map clearly reveals the presence of, three glucosyl units of the inhibitor, consistent with the known number of, sugar-binding subsites, located at the active site of the enzyme in the, -2, -1, and +1 subsites, i.e., actually spanning the point of enzymatic, cleavage. The pyranose ring at the point of potential enzymatic cleavage, is ... [[http://ispc.weizmann.ac.il/pmbin/getpm?8952478 (full description)]]
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<StructureSection load='1ovw' size='340' side='right'caption='[[1ovw]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ovw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OVW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OVW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=PRD_900097:4-thio-beta-D-glucopyranosyl-(1- 4)-4-thio-beta-D-glucopyranosyl-(1- 4)-1,4-dithio-beta-D-glucopyranose'>PRD_900097</scene>, <scene name='pdbligand=SGC:4-DEOXY-4-THIO-BETA-D-GLUCOPYRANOSE'>SGC</scene>, <scene name='pdbligand=SSG:1,4-DEOXY-1,4-DITHIO-BETA-D-GLUCOPYRANOSE'>SSG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ovw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ovw OCA], [https://pdbe.org/1ovw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ovw RCSB], [https://www.ebi.ac.uk/pdbsum/1ovw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ovw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GUNC_FUSOX GUNC_FUSOX]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ov/1ovw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ovw ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1OVW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]] with NAG and DP5 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Cellulase Cellulase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]]. Structure known Active Sites: CTA, CTB, CTC and CTD. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OVW OCA]].
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of the Fusarium oxysporum endoglucanase I with a nonhydrolyzable substrate analogue: substrate distortion gives rise to the preferred axial orientation for the leaving group., Sulzenbacher G, Driguez H, Henrissat B, Schulein M, Davies GJ, Biochemistry. 1996 Dec 3;35(48):15280-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8952478 8952478]
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[[Category: Cellulase]]
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[[Category: Fusarium oxysporum]]
[[Category: Fusarium oxysporum]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Davies, G.J.]]
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[[Category: Davies GJ]]
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[[Category: Schulein, M.]]
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[[Category: Schulein M]]
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[[Category: Sulzenbacher, G.]]
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[[Category: Sulzenbacher G]]
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[[Category: DP5]]
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[[Category: NAG]]
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[[Category: cellulose degradation]]
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[[Category: glycoprotein]]
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[[Category: glycosidase]]
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[[Category: hydrolase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:58:28 2007''
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Current revision

ENDOGLUCANASE I COMPLEXED WITH NON-HYDROLYSABLE SUBSTRATE ANALOGUE

PDB ID 1ovw

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