1pfo

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[[Image:1pfo.gif|left|200px]]<br />
 
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<applet load="1pfo" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pfo, resolution 2.2&Aring;" />
 
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'''PERFRINGOLYSIN O'''<br />
 
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==Overview==
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==PERFRINGOLYSIN O==
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The mechanisms by which proteins gain entry into membranes is a, fundamental problem in biology. Here, we present the first crystal, structure of a thiol-activated cytolysin, perfringolysin O, a member of a, large family of toxins that kill eukaryotic cells by punching holes in, their membranes. The molecule adopts an unusually elongated shape rich in, beta sheet. We have used electron microscopy data to construct a detailed, model of the membrane channel form of the toxin. The structures reveal a, novel mechanism for membrane insertion. Surprisingly, the toxin receptor, cholesterol, appears to play multiple roles: targeting, promotion of, oligomerization, triggering a membrane insertion competent form, and, stabilizing the membrane pore.
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<StructureSection load='1pfo' size='340' side='right'caption='[[1pfo]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1pfo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PFO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pfo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pfo OCA], [https://pdbe.org/1pfo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pfo RCSB], [https://www.ebi.ac.uk/pdbsum/1pfo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pfo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TACY_CLOPE TACY_CLOPE] Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A conformation change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host cell membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation.<ref>PMID:17328912</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pf/1pfo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pfo ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1PFO is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]]. Structure known Active Site: ACT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PFO OCA]].
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*[[Cytolysin 3D structures|Cytolysin 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structure of a cholesterol-binding, thiol-activated cytolysin and a model of its membrane form., Rossjohn J, Feil SC, McKinstry WJ, Tweten RK, Parker MW, Cell. 1997 May 30;89(5):685-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9182756 9182756]
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__TOC__
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</StructureSection>
[[Category: Clostridium perfringens]]
[[Category: Clostridium perfringens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Parker, M.W.]]
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[[Category: Parker MW]]
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[[Category: Rossjohn, J.]]
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[[Category: Rossjohn J]]
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[[Category: cytolysis]]
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[[Category: hemolysis]]
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[[Category: thiol-activated cytolysin]]
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[[Category: toxin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:58:46 2007''
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Current revision

PERFRINGOLYSIN O

PDB ID 1pfo

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