2ej1
From Proteopedia
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| - | [[Image:2ej1.jpg|left|200px]]<br /><applet load="2ej1" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="2ej1, resolution 1.80Å" /> | ||
| - | '''Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum'''<br /> | ||
| - | == | + | ==Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum== |
| - | + | <StructureSection load='2ej1' size='340' side='right'caption='[[2ej1]], [[Resolution|resolution]] 1.80Å' scene=''> | |
| - | [ | + | == Structural highlights == |
| - | + | <table><tr><td colspan='2'>[[2ej1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus Acetivibrio thermocellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EJ1 FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | |
| - | [ | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRD_900020:beta-cellohexaose'>PRD_900020</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ej1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ej1 OCA], [https://pdbe.org/2ej1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ej1 RCSB], [https://www.ebi.ac.uk/pdbsum/2ej1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ej1 ProSAT]</span></td></tr> | |
| - | [ | + | </table> |
| - | [[ | + | == Function == |
| - | + | [https://www.uniprot.org/uniprot/A3DD30_ACET2 A3DD30_ACET2] | |
| - | + | == Evolutionary Conservation == | |
| - | + | [[Image:Consurf_key_small.gif|200px|right]] | |
| - | + | Check<jmol> | |
| - | + | <jmolCheckbox> | |
| - | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ej/2ej1_consurf.spt"</scriptWhenChecked> | |
| - | [ | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | [[ | + | <text>to colour the structure by Evolutionary Conservation</text> |
| - | + | </jmolCheckbox> | |
| - | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ej1 ConSurf]. | |
| - | + | <div style="clear:both"></div> | |
| - | + | <div style="background-color:#fffaf0;"> | |
| - | + | == Publication Abstract from PubMed == | |
| + | The crystal structure of Cel44A, which is one of the enzymatic components of the cellulosome of Clostridium thermocellum, was solved at a resolution of 0.96 A. This enzyme belongs to glycoside hydrolase family (GH family) 44. The structure reveals that Cel44A consists of a TIM-like barrel domain and a beta-sandwich domain. The wild-type and the E186Q mutant structures complexed with substrates suggest that two glutamic acid residues, Glu(186) and Glu(359), are the active residues of the enzyme. Biochemical experiments were performed to confirm this idea. The structural features indicate that GH family 44 belongs to clan GH-A and that the reaction catalyzed by Cel44A is retaining type hydrolysis. The stereochemical course of hydrolysis was confirmed by a (1)H NMR experiment using the reduced cellooligosaccharide as a substrate. | ||
| - | + | Crystal structure of Cel44A, a glycoside hydrolase family 44 endoglucanase from Clostridium thermocellum.,Kitago Y, Karita S, Watanabe N, Kamiya M, Aizawa T, Sakka K, Tanaka I J Biol Chem. 2007 Dec 7;282(49):35703-11. Epub 2007 Sep 28. PMID:17905739<ref>PMID:17905739</ref> | |
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 2ej1" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Glucanase 3D structures|Glucanase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Acetivibrio thermocellus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Karita S]] | ||
| + | [[Category: Kitago Y]] | ||
| + | [[Category: Sakka K]] | ||
| + | [[Category: Tanaka I]] | ||
| + | [[Category: Watanabe N]] | ||
Current revision
Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum
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