3zfh

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{{STRUCTURE_3zfh| PDB=3zfh | SCENE= }}
 
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===Crystal structure of Pseudomonas aeruginosa inosine 5'-monophosphate dehydrogenase===
 
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{{ABSTRACT_PUBMED_23519796}}
 
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==Function==
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==Crystal structure of Pseudomonas aeruginosa inosine 5'-monophosphate dehydrogenase==
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[[http://www.uniprot.org/uniprot/Q9HXM5_PSEAE Q9HXM5_PSEAE]] Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth (By similarity).[HAMAP-Rule:MF_01964]
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<StructureSection load='3zfh' size='340' side='right'caption='[[3zfh]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3zfh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZFH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zfh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zfh OCA], [https://pdbe.org/3zfh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zfh RCSB], [https://www.ebi.ac.uk/pdbsum/3zfh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zfh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9HXM5_PSEAE Q9HXM5_PSEAE] Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth (By similarity).[HAMAP-Rule:MF_01964]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Inosine 5'-monophosphate dehydrogenase (IMPDH) represents a potential antimicrobial drug target. The crystal structure of recombinant Pseudomonas aeruginosa IMPDH has been determined to a resolution of 2.25 A. The structure is a homotetramer of subunits dominated by a (beta/alpha)8-barrel fold, consistent with other known structures of IMPDH. Also in common with previous work, the cystathionine beta-synthase domains, residues 92-204, are not present in the model owing to disorder. However, unlike the majority of available structures, clearly defined electron density exists for a loop that creates part of the active site. This loop, composed of residues 297-315, links alpha8 and beta9 and carries the catalytic Cys304. P. aeruginosa IMPDH shares a high level of sequence identity with bacterial and protozoan homologues, with residues involved in binding substrate and the NAD(+) cofactor being conserved. Specific differences that have been proven to contribute to selectivity against the human enzyme in a study of Cryptosporidium parvum IMPDH are also conserved, highlighting the potential value of IMPDH as a drug target.
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==About this Structure==
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Structure of Pseudomonas aeruginosa inosine 5'-monophosphate dehydrogenase.,Rao VA, Shepherd SM, Owen R, Hunter WN Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Mar 1;69(Pt 3):243-7. doi:, 10.1107/S1744309113002352. Epub 2013 Feb 22. PMID:23519796<ref>PMID:23519796</ref>
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[[3zfh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZFH OCA].
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[[Category: IMP dehydrogenase]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Pseudomonas aeruginosa pao1]]
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</div>
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[[Category: Hunter, W N.]]
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<div class="pdbe-citations 3zfh" style="background-color:#fffaf0;"></div>
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[[Category: Owen, R.]]
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[[Category: Rao, V A.]]
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==See Also==
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[[Category: Shepherd, S M.]]
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*[[Inosine monophosphate dehydrogenase 3D structures|Inosine monophosphate dehydrogenase 3D structures]]
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[[Category: Guanine nucleotide biosynthesis]]
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== References ==
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[[Category: Oxidoreductase]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Hunter WN]]
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[[Category: Owen R]]
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[[Category: Rao VA]]
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[[Category: Shepherd SM]]

Current revision

Crystal structure of Pseudomonas aeruginosa inosine 5'-monophosphate dehydrogenase

PDB ID 3zfh

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