4ji0

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'''Unreleased structure'''
 
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The entry 4ji0 is ON HOLD until Paper Publication
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==Crystal Structure of 30S ribosomal subunit from Thermus thermophilus==
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<StructureSection load='4ji0' size='340' side='right'caption='[[4ji0]], [[Resolution|resolution]] 3.49&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4ji0]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JI0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JI0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.492&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0TD:(3S)-3-(METHYLSULFANYL)-L-ASPARTIC+ACID'>0TD</scene>, <scene name='pdbligand=2MG:2N-METHYLGUANOSINE-5-MONOPHOSPHATE'>2MG</scene>, <scene name='pdbligand=4OC:4N,O2-METHYLCYTIDINE-5-MONOPHOSPHATE'>4OC</scene>, <scene name='pdbligand=5MC:5-METHYLCYTIDINE-5-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=M2G:N2-DIMETHYLGUANOSINE-5-MONOPHOSPHATE'>M2G</scene>, <scene name='pdbligand=MA6:6N-DIMETHYLADENOSINE-5-MONOPHOSHATE'>MA6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=UR3:3-METHYLURIDINE-5-MONOPHOSHATE'>UR3</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ji0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ji0 OCA], [https://pdbe.org/4ji0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ji0 RCSB], [https://www.ebi.ac.uk/pdbsum/4ji0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ji0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS4_THET8 RS4_THET8] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the body and platform of the 30S subunit. Binds mRNA in the 70S ribosome, positioning it for translation.[HAMAP-Rule:MF_01306_B]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The ribosome decodes mRNA by monitoring the geometry of codon-anticodon base-pairing using a set of universally conserved 16S rRNA nucleotides within the conformationally dynamic decoding site. By applying single-molecule FRET and X-ray crystallography, we have determined that conditional-lethal, streptomycin-dependence mutations in ribosomal protein S12 interfere with tRNA selection by allowing conformational distortions of the decoding site that impair GTPase activation of EF-Tu during the tRNA selection process. Distortions in the decoding site are reversed by streptomycin or by a second-site suppressor mutation in 16S rRNA. These observations encourage a refinement of the current model for decoding, wherein ribosomal protein S12 and the decoding site collaborate to optimize codon recognition and substrate discrimination during the early stages of the tRNA selection process.
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Authors: Demirci, H., Wang, L., Murphy IV, F., Murphy, E., Carr, J., Blanchard, S., Jogl, G., Dahlberg, A.E., Gregory, S.T.
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The central role of protein S12 in organizing the structure of the decoding site of the ribosome.,Demirci H, Wang L, Murphy FV 4th, Murphy EL, Carr JF, Blanchard SC, Jogl G, Dahlberg AE, Gregory ST RNA. 2013 Oct 23. PMID:24152548<ref>PMID:24152548</ref>
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Description: Crystal Structure of 30S ribosomal subunit from Thermus thermophilus
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4ji0" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Blanchard S]]
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[[Category: Carr J]]
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[[Category: Dahlberg AE]]
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[[Category: Demirci H]]
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[[Category: Gregory ST]]
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[[Category: Jogl G]]
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[[Category: Murphy E]]
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[[Category: Murphy IV F]]
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[[Category: Wang L]]

Current revision

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

PDB ID 4ji0

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