3vx4

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{{STRUCTURE_3vx4| PDB=3vx4 | SCENE= }}
 
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===Crystal Structure of the Nucleotide-Binding Domain of S. mutans ComA, a Bifunctional ATP-binding Cassette Transporter Involved in the Quorum-sensing Pathway===
 
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{{ABSTRACT_PUBMED_23534432}}
 
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==About this Structure==
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==Crystal Structure of the Nucleotide-Binding Domain of S. mutans ComA, a Bifunctional ATP-binding Cassette Transporter Involved in the Quorum-sensing Pathway==
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[[3vx4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_mutans_ua159 Streptococcus mutans ua159]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VX4 OCA].
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<StructureSection load='3vx4' size='340' side='right'caption='[[3vx4]], [[Resolution|resolution]] 2.69&Aring;' scene=''>
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[[Category: Streptococcus mutans ua159]]
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== Structural highlights ==
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[[Category: Hayashi, H.]]
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<table><tr><td colspan='2'>[[3vx4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_mutans_UA159 Streptococcus mutans UA159]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VX4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VX4 FirstGlance]. <br>
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[[Category: Ishii, S.]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.69&#8491;</td></tr>
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[[Category: Murakawa, T.]]
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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[[Category: Okamoto, A.]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vx4 OCA], [https://pdbe.org/3vx4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vx4 RCSB], [https://www.ebi.ac.uk/pdbsum/3vx4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vx4 ProSAT]</span></td></tr>
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[[Category: Yano, T.]]
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</table>
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[[Category: Abc transporter]]
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== Function ==
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[[Category: Atp binding]]
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[https://www.uniprot.org/uniprot/Q8DW05_STRMU Q8DW05_STRMU]
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[[Category: Transport protein]]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The ATP-binding cassette (ABC) transporter ComA is a key molecule essential for the first step of the quorum-sensing system of Streptococcus. The nucleotide binding domains (NBD) of Streptococcus mutans ComA with different N termini, NBD1 (amino acid residues 495-760), NBD2 (517-760), and NBD3 (528-760), were expressed, purified, and characterized. The shortest NBD3 corresponds to the region commonly defined as NBD in the database searches of ABC transporters. A kinetic analysis showed that the extra N-terminal region conferred a significantly higher ATP hydrolytic activity on the NBD at a neutral pH. Gel-filtration, X-ray crystallography, and mutational analyses suggest that at least four to five residues beyond the N-terminal boundary of NBD3 indeed participate in stabilizing the protein scaffold of the domain structure, thereby facilitating the ATP-dependent dimerization of NBD which is a prerequisite to the catalysis. These findings, together with the presence of a highly conserved glycine residue in this region, support the redefinition of the N-terminal boundary of the NBD of these types of ABC exporters.
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Boundary of the Nucleotide-Binding Domain of Streptococcus ComA Based on Functional and Structural Analysis.,Ishii S, Yano T, Okamoto A, Murakawa T, Hayashi H Biochemistry. 2013 Apr 16;52(15):2545-55. doi: 10.1021/bi3017069. Epub 2013 Apr, 5. PMID:23534432<ref>PMID:23534432</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3vx4" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Streptococcus mutans UA159]]
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[[Category: Hayashi H]]
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[[Category: Ishii S]]
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[[Category: Murakawa T]]
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[[Category: Okamoto A]]
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[[Category: Yano T]]

Current revision

Crystal Structure of the Nucleotide-Binding Domain of S. mutans ComA, a Bifunctional ATP-binding Cassette Transporter Involved in the Quorum-sensing Pathway

PDB ID 3vx4

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