3n7l
From Proteopedia
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| - | {{STRUCTURE_3n7l| PDB=3n7l | SCENE= }} | ||
| - | ===Crystal structure of botulinum neurotoxin serotype D/C VPI 5993 binding domain=== | ||
| - | {{ABSTRACT_PUBMED_20731382}} | ||
| - | == | + | ==Crystal structure of botulinum neurotoxin serotype D/C VPI 5993 binding domain== |
| - | [[3n7l]] is a 1 chain structure with sequence from [ | + | <StructureSection load='3n7l' size='340' side='right'caption='[[3n7l]], [[Resolution|resolution]] 2.00Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3n7l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N7L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N7L FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n7l OCA], [https://pdbe.org/3n7l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n7l RCSB], [https://www.ebi.ac.uk/pdbsum/3n7l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n7l ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9LBR1_CLOBO Q9LBR1_CLOBO] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n7/3n7l_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n7l ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The botulinum neurotoxins (BoNTs) are the most potent protein toxins for humans. There are seven serotypes of BoNTs (A-G) based on a lack of cross antiserum neutralization. BoNTs utilize gangliosides as components of the host receptors for binding and entry into neurons. Members of BoNT/C and BoNT/D serotypes include mosaic toxins that are organized in D/C and C/D toxins. One D/C mosaic toxin, BoNT/D-South Africa (BoNT/D-SA), was not fully neutralized by immunization with BoNT serotype C or D, which stimulated this study. Here the crystal structures of the receptor binding domains of BoNT/C, BoNT/D, and BoNT/D-SA are presented. Biochemical and cell binding studies show that BoNT/C and BoNT/D-SA possess unique mechanisms for ganglioside binding. These studies provide new information about how the BoNTs can enter host cells as well as a basis for understanding the immunological diversity of these neurotoxins. | ||
| - | + | Identification of a Unique Ganglioside Binding Loop within Botulinum Neurotoxins C and D-SA .,Karalewitz AP, Kroken AR, Fu Z, Baldwin MR, Kim JJ, Barbieri JT Biochemistry. 2010 Aug 23. PMID:20731382<ref>PMID:20731382</ref> | |
| - | <ref | + | |
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3n7l" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Clostridium botulinum]] | [[Category: Clostridium botulinum]] | ||
| - | [[Category: Baldwin | + | [[Category: Large Structures]] |
| - | [[Category: Barbieri | + | [[Category: Baldwin MR]] |
| - | [[Category: Fu | + | [[Category: Barbieri JT]] |
| - | [[Category: Karalewitz | + | [[Category: Fu Z]] |
| - | [[Category: Kim | + | [[Category: Karalewitz A]] |
| - | [[Category: Kroken | + | [[Category: Kim J-JP]] |
| - | + | [[Category: Kroken A]] | |
| - | + | ||
| - | + | ||
| - | + | ||
Current revision
Crystal structure of botulinum neurotoxin serotype D/C VPI 5993 binding domain
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