3mp2

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{{STRUCTURE_3mp2| PDB=3mp2 | SCENE= }}
 
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===Crystal structure of transmissible gastroenteritis virus papain-like protease 1===
 
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{{ABSTRACT_PUBMED_20668092}}
 
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==Function==
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==Crystal structure of transmissible gastroenteritis virus papain-like protease 1==
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[[http://www.uniprot.org/uniprot/R1A_CVPPU R1A_CVPPU]] The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity). The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SAGC]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity).
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<StructureSection load='3mp2' size='340' side='right'caption='[[3mp2]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[3mp2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Porcine_transmissible_gastroenteritis_coronavirus_strain_Purdue Porcine transmissible gastroenteritis coronavirus strain Purdue]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MP2 FirstGlance]. <br>
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[[3mp2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Transmissible_gastroenteritis_virus Transmissible gastroenteritis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MP2 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mp2 OCA], [https://pdbe.org/3mp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mp2 RCSB], [https://www.ebi.ac.uk/pdbsum/3mp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mp2 ProSAT]</span></td></tr>
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<ref group="xtra">PMID:020668092</ref><references group="xtra"/><references/>
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</table>
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[[Category: Transmissible gastroenteritis virus]]
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== Function ==
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[[Category: Manolaridis, I.]]
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[https://www.uniprot.org/uniprot/R1A_CVPPU R1A_CVPPU] The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity). The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SAGC]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity).
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[[Category: Tucker, P A.]]
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== Evolutionary Conservation ==
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[[Category: Wojdyla, J A.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Coronavirus]]
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Check<jmol>
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[[Category: Hydrolase]]
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<jmolCheckbox>
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[[Category: Papain-like protease]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mp/3mp2_consurf.spt"</scriptWhenChecked>
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[[Category: Tgev]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mp2 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Porcine transmissible gastroenteritis coronavirus strain Purdue]]
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[[Category: Manolaridis I]]
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[[Category: Tucker PA]]
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[[Category: Wojdyla JA]]

Current revision

Crystal structure of transmissible gastroenteritis virus papain-like protease 1

PDB ID 3mp2

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