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| - | {{STRUCTURE_3ml3| PDB=3ml3 | SCENE= }} | |
| - | ===Crystal structure of the IcsA autochaperone region=== | |
| - | {{ABSTRACT_PUBMED_21335457}} | |
| | | | |
| - | ==Function== | + | ==Crystal structure of the IcsA autochaperone region== |
| - | [[http://www.uniprot.org/uniprot/ICSA_SHIFL ICSA_SHIFL]] Essential for bacterial spreading by eliciting polar deposition of filamentous actin (actin-based motility). Inside the host cell mediates nucleation and polymerization of actin molecules on the bacterial surface, which provides the propulsive force for intracellular movement and intercellular dissemination of the bacterium. During invasion of mammalian cells, triggers autophagy by binding to APG5L. Interaction with IcsB leads to escape from the autophagic host defense system. Also binds ATP and displays weak ATPase activity.<ref>PMID:7896693</ref> <ref>PMID:2542950</ref> <ref>PMID:1602963</ref> <ref>PMID:9582270</ref> <ref>PMID:15576571</ref> | + | <StructureSection load='3ml3' size='340' side='right'caption='[[3ml3]], [[Resolution|resolution]] 2.00Å' scene=''> |
| - | | + | == Structural highlights == |
| - | ==About this Structure== | + | <table><tr><td colspan='2'>[[3ml3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ML3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ML3 FirstGlance]. <br> |
| - | [[3ml3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ML3 OCA].
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ml3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ml3 OCA], [https://pdbe.org/3ml3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ml3 RCSB], [https://www.ebi.ac.uk/pdbsum/3ml3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ml3 ProSAT]</span></td></tr> |
| - | ==Reference==
| + | </table> |
| - | <ref group="xtra">PMID:021335457</ref><references group="xtra"/><references/>
| + | == Function == |
| | + | [https://www.uniprot.org/uniprot/ICSA_SHIFL ICSA_SHIFL] Essential for bacterial spreading by eliciting polar deposition of filamentous actin (actin-based motility). Inside the host cell mediates nucleation and polymerization of actin molecules on the bacterial surface, which provides the propulsive force for intracellular movement and intercellular dissemination of the bacterium. During invasion of mammalian cells, triggers autophagy by binding to APG5L. Interaction with IcsB leads to escape from the autophagic host defense system. Also binds ATP and displays weak ATPase activity.<ref>PMID:7896693</ref> <ref>PMID:2542950</ref> <ref>PMID:1602963</ref> <ref>PMID:9582270</ref> <ref>PMID:15576571</ref> |
| | + | == References == |
| | + | <references/> |
| | + | __TOC__ |
| | + | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Shigella flexneri]] | | [[Category: Shigella flexneri]] |
| - | [[Category: Diezmann, D.]] | + | [[Category: Diezmann D]] |
| - | [[Category: Kuhnel, K.]] | + | [[Category: Kuhnel K]] |
| - | [[Category: Beta helix]]
| + | |
| - | [[Category: Beta sandwich]]
| + | |
| - | [[Category: Protein transport]]
| + | |
| Structural highlights
Function
ICSA_SHIFL Essential for bacterial spreading by eliciting polar deposition of filamentous actin (actin-based motility). Inside the host cell mediates nucleation and polymerization of actin molecules on the bacterial surface, which provides the propulsive force for intracellular movement and intercellular dissemination of the bacterium. During invasion of mammalian cells, triggers autophagy by binding to APG5L. Interaction with IcsB leads to escape from the autophagic host defense system. Also binds ATP and displays weak ATPase activity.[1] [2] [3] [4] [5]
References
- ↑ Fukuda I, Suzuki T, Munakata H, Hayashi N, Katayama E, Yoshikawa M, Sasakawa C. Cleavage of Shigella surface protein VirG occurs at a specific site, but the secretion is not essential for intracellular spreading. J Bacteriol. 1995 Apr;177(7):1719-26. PMID:7896693
- ↑ Bernardini ML, Mounier J, d'Hauteville H, Coquis-Rondon M, Sansonetti PJ. Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin. Proc Natl Acad Sci U S A. 1989 May;86(10):3867-71. PMID:2542950
- ↑ d'Hauteville H, Sansonetti PJ. Phosphorylation of IcsA by cAMP-dependent protein kinase and its effect on intracellular spread of Shigella flexneri. Mol Microbiol. 1992 Apr;6(7):833-41. PMID:1602963
- ↑ Suzuki T, Miki H, Takenawa T, Sasakawa C. Neural Wiskott-Aldrich syndrome protein is implicated in the actin-based motility of Shigella flexneri. EMBO J. 1998 May 15;17(10):2767-76. PMID:9582270 doi:10.1093/emboj/17.10.2767
- ↑ Ogawa M, Yoshimori T, Suzuki T, Sagara H, Mizushima N, Sasakawa C. Escape of intracellular Shigella from autophagy. Science. 2005 Feb 4;307(5710):727-31. Epub 2004 Dec 2. PMID:15576571 doi:10.1126/science.1106036
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