3mqy
From Proteopedia
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| - | {{STRUCTURE_3mqy| PDB=3mqy | SCENE= }} | ||
| - | ===SgrAI with cleaved DNA and Magnesium bound=== | ||
| - | {{ABSTRACT_PUBMED_21206063}} | ||
| - | == | + | ==SgrAI with cleaved DNA and Magnesium bound== |
| - | [[3mqy]] is a 6 chain structure with sequence from [ | + | <StructureSection load='3mqy' size='340' side='right'caption='[[3mqy]], [[Resolution|resolution]] 2.00Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3mqy]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MQY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MQY FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mqy OCA], [https://pdbe.org/3mqy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mqy RCSB], [https://www.ebi.ac.uk/pdbsum/3mqy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mqy ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9F6L0_STRGR Q9F6L0_STRGR] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | SgrAI is a type II restriction endonuclease that cuts an unusually long recognition sequence and exhibits allosteric self-activation with expansion of DNA-sequence specificity. The three-dimensional crystal structures of SgrAI bound to cleaved primary-site DNA and Mg(2)(+) and bound to secondary-site DNA with either Mg(2)(+) or Ca(2)(+) are presented. All three structures show a conformation of enzyme and DNA similar to the previously determined dimeric structure of SgrAI bound to uncleaved primary-site DNA and Ca(2)(+) [Dunten et al. (2008), Nucleic Acids Res. 36, 5405-5416], with the exception of the cleaved bond and a slight shifting of the DNA in the SgrAI/cleaved primary-site DNA/Mg(2)(+) structure. In addition, a new metal ion binding site is located in one of the two active sites in this structure, which is consistent with proposals for the existence of a metal-ion site near the 3'-O leaving group. | ||
| - | + | New clues in the allosteric activation of DNA cleavage by SgrAI: structures of SgrAI bound to cleaved primary-site DNA and uncleaved secondary-site DNA.,Little EJ, Dunten PW, Bitinaite J, Horton NC Acta Crystallogr D Biol Crystallogr. 2011 Jan;67(Pt 1):67-74. Epub 2010, Dec 16. PMID:21206063<ref>PMID:21206063</ref> | |
| - | <ref | + | |
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3mqy" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Streptomyces griseus]] | [[Category: Streptomyces griseus]] | ||
| - | + | [[Category: Dunten PW]] | |
| - | [[Category: Dunten | + | [[Category: Horton NC]] |
| - | [[Category: Horton | + | [[Category: Little EJ]] |
| - | [[Category: Little | + | |
| - | + | ||
| - | + | ||
Current revision
SgrAI with cleaved DNA and Magnesium bound
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