2evu

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[[Image:2evu.gif|left|200px]]<br /><applet load="2evu" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2evu, resolution 2.3&Aring;" />
 
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'''Crystal structure of aquaporin AqpM at 2.3A resolution'''<br />
 
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==Overview==
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==Crystal structure of aquaporin AqpM at 2.3A resolution==
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To explore the structural basis of the unique selectivity spectrum and conductance of the transmembrane channel protein AqpM from the archaeon Methanothermobacter marburgensis, we determined the structure of AqpM to 1.68-A resolution by x-ray crystallography. The structure establishes AqpM as being in a unique subdivision between the two major subdivisions of aquaporins, the water-selective aquaporins, and the water-plus-glycerol-conducting aquaglyceroporins. In AqpM, isoleucine replaces a key histidine residue found in the lumen of water channels, which becomes a glycine residue in aquaglyceroporins. As a result of this and other side-chain substituents in the walls of the channel, the channel is intermediate in size and exhibits differentially tuned electrostatics when compared with the other subfamilies.
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<StructureSection load='2evu' size='340' side='right'caption='[[2evu]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2evu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_marburgensis_str._Marburg Methanothermobacter marburgensis str. Marburg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EVU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2evu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2evu OCA], [https://pdbe.org/2evu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2evu RCSB], [https://www.ebi.ac.uk/pdbsum/2evu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2evu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AQPM_METTM AQPM_METTM] Channel that permits osmotically driven movement of water in both directions. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity. Exhibits also a transient but reproducible increase in the initial glycerol flux.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ev/2evu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2evu ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2EVU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus] with <scene name='pdbligand=BOG:'>BOG</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EVU OCA].
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*[[Aquaporin 3D structures|Aquaporin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural basis for conductance by the archaeal aquaporin AqpM at 1.68 A., Lee JK, Kozono D, Remis J, Kitagawa Y, Agre P, Stroud RM, Proc Natl Acad Sci U S A. 2005 Dec 27;102(52):18932-7. Epub 2005 Dec 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16361443 16361443]
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[[Category: Large Structures]]
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[[Category: Methanothermobacter thermautotrophicus]]
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[[Category: Methanothermobacter marburgensis str. Marburg]]
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[[Category: Single protein]]
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[[Category: Agre P]]
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[[Category: Agre, P.]]
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[[Category: Kitagawa Y]]
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[[Category: Kitagawa, Y.]]
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[[Category: Kozono D]]
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[[Category: Kozono, D.]]
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[[Category: Lee JK]]
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[[Category: Lee, J K.]]
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[[Category: Remis J]]
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[[Category: Remis, J.]]
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[[Category: Stroud RM]]
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[[Category: Stroud, R M.]]
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[[Category: BOG]]
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[[Category: GOL]]
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[[Category: alpha-helical]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:15:02 2008''
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Current revision

Crystal structure of aquaporin AqpM at 2.3A resolution

PDB ID 2evu

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