This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1qmf

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:04, 17 April 2024) (edit) (undo)
 
(24 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1qmf.gif|left|200px]]<br />
 
-
<applet load="1qmf" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1qmf, resolution 2.80&Aring;" />
 
-
'''PENICILLIN-BINDING PROTEIN 2X (PBP-2X) ACYL-ENZYME COMPLEX'''<br />
 
-
==Overview==
+
==PENICILLIN-BINDING PROTEIN 2X (PBP-2X) ACYL-ENZYME COMPLEX==
-
Penicillin-binding proteins (PBPs), the primary targets for beta-lactam, antibiotics, are periplasmic membrane-attached proteins responsible for, the construction and maintenance of the bacterial cell wall. Bacteria have, developed several mechanisms of resistance, one of which is the mutation, of the target enzymes to reduce their affinity for beta-lactam, antibiotics. Here, we describe the structure of PBP2x from Streptococcus, pneumoniae determined to 2.4 A. In addition, we also describe the PBP2x, structure in complex with cefuroxime, a therapeutically relevant, antibiotic, at 2.8 A. Surprisingly, two antibiotic molecules are observed:, one as a covalent complex with the active-site serine residue, and a, second one between the C-terminal and the transpeptidase domains. The, structure ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10860753 (full description)]]
+
<StructureSection load='1qmf' size='340' side='right'caption='[[1qmf]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1qmf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QMF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QMF FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CES:2-[CARBOXY-(2-FURAN-2-YL-2-METHOXYIMINO-ACETYLAMINO)-METHYL]-5-METHYL-3,6-DIHYDRO-2H-[1,3]THIAZINE-4-CARBOXYLIC+ACID'>CES</scene>, <scene name='pdbligand=KEF:CEFUROXIME+(OCT-3-ENE+FORM)'>KEF</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qmf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qmf OCA], [https://pdbe.org/1qmf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qmf RCSB], [https://www.ebi.ac.uk/pdbsum/1qmf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qmf ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PBPX_STRPN PBPX_STRPN] Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. Beta-lactams inactivate the PBPs by acylating an essential serine residue in the active site of these proteins.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qm/1qmf_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qmf ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1QMF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]] with CES and KEF as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: SER. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QMF OCA]].
+
*[[Penicillin-binding protein 3D structures|Penicillin-binding protein 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
The crystal structure of the penicillin-binding protein 2x from Streptococcus pneumoniae and its acyl-enzyme form: implication in drug resistance., Gordon E, Mouz N, Duee E, Dideberg O, J Mol Biol. 2000 Jun 2;299(2):477-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10860753 10860753]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Streptococcus pneumoniae]]
[[Category: Streptococcus pneumoniae]]
-
[[Category: Dideberg, O.]]
+
[[Category: Dideberg O]]
-
[[Category: Duee, E.]]
+
[[Category: Duee E]]
-
[[Category: Gordon, E.J.]]
+
[[Category: Gordon EJ]]
-
[[Category: Mouz, N.]]
+
[[Category: Mouz N]]
-
[[Category: CES]]
+
-
[[Category: KEF]]
+
-
[[Category: cell wall]]
+
-
[[Category: peptidoglycan synthesis]]
+
-
[[Category: resistance]]
+
-
[[Category: transmembrane]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:01:57 2007''
+

Current revision

PENICILLIN-BINDING PROTEIN 2X (PBP-2X) ACYL-ENZYME COMPLEX

PDB ID 1qmf

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qmf"
Personal tools