2f8u

[[Image:2f8u.gif|left|200px]]<br /><applet load="2f8u" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2f8u" />

'''Solution structure of G-quadruplex formed in the human Bcl-2 promoter'''<br />

==Overview==

BCL2 protein functions as an inhibitor of cell apoptosis and has been found to be aberrantly expressed in a wide range of human diseases. A highly GC-rich region upstream of the P1 promoter plays an important role in the transcriptional regulation of BCL2. Here we report the NMR solution structure of the major intramolecular G-quadruplex formed on the G-rich strand of this region in K+ solution. This well-defined mixed parallel/antiparallel-stranded G-quadruplex structure contains three G-tetrads of mixed G-arrangements, which are connected with two lateral loops and one side loop, and four grooves of different widths. The three loops interact with the core G-tetrads in a specific way that defines and stabilizes the overall G-quadruplex structure. The loop conformations are in accord with the experimental mutation and footprinting data. The first 3-nt loop adopts a lateral loop conformation and appears to determine the overall folding of the BCL2 G-quadruplex. The third 1-nt double-chain-reversal loop defines another example of a stable parallel-stranded structural motif using the G3NG3 sequence. Significantly, the distinct major BCL2 promoter G-quadruplex structure suggests that it can be specifically involved in gene modulation and can be an attractive target for pathway-specific drug design.

 

==About this Structure==

2F8U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8U OCA].

 

==Reference==

NMR solution structure of the major G-quadruplex structure formed in the human BCL2 promoter region., Dai J, Chen D, Jones RA, Hurley LH, Yang D, Nucleic Acids Res. 2006;34(18):5133-44. Epub 2006 Sep 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16998187 16998187]

[[Category: Protein complex]]

[[Category: Carver, M.]]

[[Category: Chen, D.]]

[[Category: Dai, J.]]

[[Category: Yang, D.]]

[[Category: bcl-2 promoter]]

[[Category: dna]]

[[Category: g-quadruplex]]

[[Category: nmr]]