2fca

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[[Image:2fca.gif|left|200px]]<br /><applet load="2fca" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fca, resolution 2.1&Aring;" />
 
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'''The structure of BsTrmB'''<br />
 
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==Overview==
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==The structure of BsTrmB==
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The structure of Bacillus subtilis TrmB (BsTrmB), the tRNA (m7G46) methyltransferase, was determined at a resolution of 2.1 A. This is the first structure of a member of the TrmB family to be determined by X-ray crystallography. It reveals a unique variant of the Rossmann-fold methyltransferase (RFM) structure, with the N-terminal helix folded on the opposite site of the catalytic domain. The architecture of the active site and a computational docking model of BsTrmB in complex with the methyl group donor S-adenosyl-L-methionine and the tRNA substrate provide an explanation for results from mutagenesis studies of an orthologous enzyme from Escherichia coli (EcTrmB). However, unlike EcTrmB, BsTrmB is shown here to be dimeric both in the crystal and in solution. The dimer interface has a hydrophobic core and buries a potassium ion and five water molecules. The evolutionary analysis of the putative interface residues in the TrmB family suggests that homodimerization may be a specific feature of TrmBs from Bacilli, which may represent an early stage of evolution to an obligatory dimer.
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<StructureSection load='2fca' size='340' side='right'caption='[[2fca]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2fca]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FCA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fca OCA], [https://pdbe.org/2fca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fca RCSB], [https://www.ebi.ac.uk/pdbsum/2fca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fca ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRMB_BACSU TRMB_BACSU] Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.[HAMAP-Rule:MF_01057]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fc/2fca_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fca ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2FCA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/tRNA_(guanine-N(7)-)-methyltransferase tRNA (guanine-N(7)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.33 2.1.1.33] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCA OCA].
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*[[TRNA methyltransferase 3D structures|TRNA methyltransferase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of Bacillus subtilis TrmB, the tRNA (m7G46) methyltransferase., Zegers I, Gigot D, van Vliet F, Tricot C, Aymerich S, Bujnicki JM, Kosinski J, Droogmans L, Nucleic Acids Res. 2006 Apr 5;34(6):1925-34. Print 2006. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16600901 16600901]
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[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: tRNA (guanine-N(7)-)-methyltransferase]]
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[[Category: Bujnicki J]]
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[[Category: Bujnicki, J.]]
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[[Category: Droogmans L]]
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[[Category: Droogmans, L.]]
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[[Category: Gigot D]]
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[[Category: Gigot, D.]]
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[[Category: Kosinski J]]
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[[Category: Kosinski, J.]]
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[[Category: Van Vliet F]]
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[[Category: Vliet, F Van.]]
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[[Category: Zegers I]]
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[[Category: Zegers, I.]]
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[[Category: K]]
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[[Category: methyltransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:19:54 2008''
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Current revision

The structure of BsTrmB

PDB ID 2fca

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