2ffl

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[[Image:2ffl.gif|left|200px]]<br /><applet load="2ffl" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ffl, resolution 3.33&Aring;" />
 
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'''Crystal Structure of Dicer from Giardia intestinalis'''<br />
 
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==Overview==
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==Crystal Structure of Dicer from Giardia intestinalis==
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The specialized ribonuclease Dicer initiates RNA interference by cleaving double-stranded RNA (dsRNA) substrates into small fragments about 25 nucleotides in length. In the crystal structure of an intact Dicer enzyme, the PAZ domain, a module that binds the end of dsRNA, is separated from the two catalytic ribonuclease III (RNase III) domains by a flat, positively charged surface. The 65 angstrom distance between the PAZ and RNase III domains matches the length spanned by 25 base pairs of RNA. Thus, Dicer itself is a molecular ruler that recognizes dsRNA and cleaves a specified distance from the helical end.
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<StructureSection load='2ffl' size='340' side='right'caption='[[2ffl]], [[Resolution|resolution]] 3.33&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2ffl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. The February 2008 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Small Interfering RNA'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2008_2 10.2210/rcsb_pdb/mom_2008_2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FFL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FFL FirstGlance]. <br>
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2FFL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FFL OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.33&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ffl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ffl OCA], [https://pdbe.org/2ffl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ffl RCSB], [https://www.ebi.ac.uk/pdbsum/2ffl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ffl ProSAT]</span></td></tr>
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Structural basis for double-stranded RNA processing by Dicer., Macrae IJ, Zhou K, Li F, Repic A, Brooks AN, Cande WZ, Adams PD, Doudna JA, Science. 2006 Jan 13;311(5758):195-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16410517 16410517]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DCL_GIAIC DCL_GIAIC] Involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. It produces 21 to 23 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs.<ref>PMID:16410517</ref>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Giardia intestinalis]]
[[Category: Giardia intestinalis]]
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[[Category: Ribonuclease III]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Adams, P D.]]
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[[Category: Small Interfering RNA]]
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[[Category: Doudna, J A.]]
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[[Category: Adams PD]]
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[[Category: MacRae, I J.]]
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[[Category: Doudna JA]]
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[[Category: MN]]
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[[Category: MacRae IJ]]
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[[Category: rna interference; rnai; paz domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:20:55 2008''
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Current revision

Crystal Structure of Dicer from Giardia intestinalis

PDB ID 2ffl

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