3p7n

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{{STRUCTURE_3p7n| PDB=3p7n | SCENE= }}
 
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===Crystal structure of light activated transcription factor El222 from Erythrobacter litoralis===
 
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{{ABSTRACT_PUBMED_21606338}}
 
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==About this Structure==
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==Crystal structure of light activated transcription factor El222 from Erythrobacter litoralis==
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[[3p7n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Erythrobacter_litoralis Erythrobacter litoralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P7N OCA].
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<StructureSection load='3p7n' size='340' side='right'caption='[[3p7n]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3p7n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Erythrobacter_litoralis_HTCC2594 Erythrobacter litoralis HTCC2594]. The March 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P7N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P7N FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p7n OCA], [https://pdbe.org/3p7n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p7n RCSB], [https://www.ebi.ac.uk/pdbsum/3p7n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p7n ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LVHTH_ERYLH LVHTH_ERYLH] Has reversible, light-dependent DNA-binding activity. Upon illumination an internal FMN-protein adduct is formed which changes the protein conformation so that the previously sequestered DNA-binding domain is free to bind DNA. Binds to sequences within in its own promoter when illuminated but not when it has been incubated in the dark.<ref>PMID:21606338</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Light-oxygen-voltage (LOV) domains are blue light-activated signaling modules integral to a wide range of photosensory proteins. Upon illumination, LOV domains form internal protein-flavin adducts that generate conformational changes which control effector function. Here we advance our understanding of LOV regulation with structural, biophysical, and biochemical studies of EL222, a light-regulated DNA-binding protein. The dark-state crystal structure reveals interactions between the EL222 LOV and helix-turn-helix domains that we show inhibit DNA binding. Solution biophysical data indicate that illumination breaks these interactions, freeing the LOV and helix-turn-helix domains of each other. This conformational change has a key functional effect, allowing EL222 to bind DNA in a light-dependent manner. Our data reveal a conserved signaling mechanism among diverse LOV-containing proteins, where light-induced conformational changes trigger activation via a conserved interaction surface.
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==Reference==
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Structural basis of photosensitivity in a bacterial light-oxygen-voltage/helix-turn-helix (LOV-HTH) DNA-binding protein.,Nash AI, McNulty R, Shillito ME, Swartz TE, Bogomolni RA, Luecke H, Gardner KH Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9449-54. Epub 2011 May 23. PMID:21606338<ref>PMID:21606338</ref>
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<ref group="xtra">PMID:021606338</ref><references group="xtra"/><references/>
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[[Category: Erythrobacter litoralis]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Luecke, H.]]
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</div>
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[[Category: McNulty, R.]]
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<div class="pdbe-citations 3p7n" style="background-color:#fffaf0;"></div>
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[[Category: Dna binding protein]]
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== References ==
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[[Category: Light-activated transcription factor]]
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<references/>
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[[Category: Lov domain]]
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__TOC__
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</StructureSection>
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[[Category: Erythrobacter litoralis HTCC2594]]
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[[Category: Large Structures]]
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[[Category: Phototropin]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Luecke H]]
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[[Category: McNulty R]]

Current revision

Crystal structure of light activated transcription factor El222 from Erythrobacter litoralis

PDB ID 3p7n

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