From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| (4 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:2fny.gif|left|200px]]<br /><applet load="2fny" size="350" color="white" frame="true" align="right" spinBox="true" | + | #REDIRECT [[3e47]] This PDB entry is obsolete and replaced by 3e47 |
| - | caption="2fny, resolution 3.00Å" />
| + | |
| - | '''Homobelactosin C bound to the yeast 20S proteasome'''<br />
| + | |
| - | | + | |
| - | ==Overview==
| + | |
| - | Most class I MHC ligands are generated from the vast majority of cellular proteins by proteolysis within the ubiquitin-proteasome pathway and are presented on the cell surface by MHC class I molecules. Here, we present the crystallographic analysis of yeast 20S proteasome in complex with the inhibitor homobelactosin C. The structure reveals a unique inhibitor-binding mode and provides information about the composition of proteasomal primed substrate-binding sites. IFN-gamma inducible substitution of proteasomal constitutive subunits by immunosubunits modulates characteristics of generated peptides, thus producing fragments with higher preference for binding to MHC class I molecules. The structural data for the proteasome:homobelactosin C complex provide an explanation for involvement of immunosubunits in antigen generation and open perspectives for rational design of ligands, inhibiting exclusively constitutive proteasomes or immunoproteasomes.
| + | |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 2FNY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ESY:'>ESY</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Proteasome_endopeptidase_complex Proteasome endopeptidase complex], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.1 3.4.25.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FNY OCA].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | Inhibitor-binding mode of homobelactosin C to proteasomes: new insights into class I MHC ligand generation., Groll M, Larionov OV, Huber R, de Meijere A, Proc Natl Acad Sci U S A. 2006 Mar 21;103(12):4576-9. Epub 2006 Mar 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16537370 16537370]
| + | |
| - | [[Category: Proteasome endopeptidase complex]]
| + | |
| - | [[Category: Protein complex]]
| + | |
| - | [[Category: Saccharomyces cerevisiae]]
| + | |
| - | [[Category: Groll, M.]]
| + | |
| - | [[Category: ESY]]
| + | |
| - | [[Category: beta sandwich structure flanked by helices]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:23:19 2008''
| + | |
Current revision
- REDIRECT 3e47 This PDB entry is obsolete and replaced by 3e47
