2fqf

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[[Image:2fqf.jpg|left|200px]]<br /><applet load="2fqf" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fqf, resolution 2.000&Aring;" />
 
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'''Crystal Structures of E. coli Laccase CueO under different copper binding situations'''<br />
 
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==Overview==
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==Crystal Structures of E. coli Laccase CueO under different copper binding situations==
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CueO protein is a hypothetical bacterial laccase and a good laccase candidate for large scale industrial application. Four CueO crystal structures were determined at different copper concentrations. Low copper occupancy in apo-CueO and slow copper reconstitution process in CueO with exogenous copper were demonstrated. These observations well explain the copper dependence of CueO oxidase activity. Structural comparison between CueO and other three fungal laccase proteins indicates that Glu106 in CueO constitutes the primary counter-work for reconstitution of the trinuclear copper site. Mutation of Glu106 to a Phe enhanced CueO oxidation activity and supported this hypothesis. In addition, an extra alpha-helix from Leu351 to Gly378 covers substrate biding pocket of CueO and might compromises the electron transfer from substrate to type I copper.
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<StructureSection load='2fqf' size='340' side='right'caption='[[2fqf]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2fqf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FQF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FQF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fqf OCA], [https://pdbe.org/2fqf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fqf RCSB], [https://www.ebi.ac.uk/pdbsum/2fqf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fqf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CUEO_ECOLI CUEO_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fq/2fqf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fqf ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2FQF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CU:'>CU</scene>, <scene name='pdbligand=C2O:'>C2O</scene> and <scene name='pdbligand=CIT:'>CIT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FQF OCA].
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*[[Blue copper oxidase CueO|Blue copper oxidase CueO]]
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*[[Blue copper oxidase CueO 3D structures|Blue copper oxidase CueO 3D structures]]
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==Reference==
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__TOC__
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Crystal structures of E. coli laccase CueO at different copper concentrations., Li X, Wei Z, Zhang M, Peng X, Yu G, Teng M, Gong W, Biochem Biophys Res Commun. 2007 Mar 2;354(1):21-6. Epub 2006 Dec 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17217912 17217912]
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Gong, W.]]
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[[Category: Gong W]]
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[[Category: Li, X.]]
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[[Category: Li X]]
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[[Category: Teng, M.]]
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[[Category: Teng M]]
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[[Category: Wei, Z.]]
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[[Category: Wei Z]]
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[[Category: Zhang, M.]]
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[[Category: Zhang M]]
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[[Category: C2O]]
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[[Category: CIT]]
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[[Category: CU]]
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[[Category: azurin-like domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:24:10 2008''
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Current revision

Crystal Structures of E. coli Laccase CueO under different copper binding situations

PDB ID 2fqf

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