2fwr

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[[Image:2fwr.gif|left|200px]]<br /><applet load="2fwr" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fwr, resolution 2.600&Aring;" />
 
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'''Structure of Archaeoglobus Fulgidis XPB'''<br />
 
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==Overview==
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==Structure of Archaeoglobus Fulgidis XPB==
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The human xeroderma pigmentosum group B (XPB) helicase is essential for transcription, nucleotide excision repair, and TFIIH functional assembly. Here, we determined crystal structures of an Archaeoglobus fulgidus XPB homolog (AfXPB) that characterize two RecA-like XPB helicase domains and discover a DNA damage recognition domain (DRD), a unique RED motif, a flexible thumb motif (ThM), and implied conformational changes within a conserved functional core. RED motif mutations dramatically reduce helicase activity, and the DRD and ThM, which flank the RED motif, appear structurally as well as functionally analogous to the MutS mismatch recognition and DNA polymerase thumb domains. Substrate specificity is altered by DNA damage, such that AfXPB unwinds dsDNA with 3' extensions, but not blunt-ended dsDNA, unless it contains a lesion, as shown for CPD or (6-4) photoproducts. Together, these results provide an unexpected mechanism of DNA unwinding with implications for XPB damage verification in nucleotide excision repair.
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<StructureSection load='2fwr' size='340' side='right'caption='[[2fwr]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2fwr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FWR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FWR FirstGlance]. <br>
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2FWR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=IPA:'>IPA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FWR OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fwr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fwr OCA], [https://pdbe.org/2fwr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fwr RCSB], [https://www.ebi.ac.uk/pdbsum/2fwr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fwr ProSAT]</span></td></tr>
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Conserved XPB core structure and motifs for DNA unwinding: implications for pathway selection of transcription or excision repair., Fan L, Arvai AS, Cooper PK, Iwai S, Hanaoka F, Tainer JA, Mol Cell. 2006 Apr 7;22(1):27-37. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16600867 16600867]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O29889_ARCFU O29889_ARCFU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fw/2fwr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fwr ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Arvai, A S.]]
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[[Category: Arvai AS]]
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[[Category: Fan, L.]]
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[[Category: Fan L]]
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[[Category: Tainer, J A.]]
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[[Category: Tainer JA]]
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[[Category: IPA]]
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[[Category: PO4]]
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[[Category: dna repair]]
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[[Category: dna unwinding]]
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[[Category: xpb]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:51 2008''
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Current revision

Structure of Archaeoglobus Fulgidis XPB

PDB ID 2fwr

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