2g1g
From Proteopedia
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| - | [[Image:2g1g.gif|left|200px]]<br /><applet load="2g1g" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="2g1g" /> | ||
| - | '''Solution structure of the anticodon loop of S. Pombe tRNAi including the naturally occurring N6-threonyl adenine'''<br /> | ||
| - | == | + | ==Solution structure of the anticodon loop of S. Pombe tRNAi including the naturally occurring N6-threonyl adenine== |
| + | <StructureSection load='2g1g' size='340' side='right'caption='[[2g1g]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2g1g]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G1G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G1G FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=T6A:N-[N-(9-B-D-RIBOFURANOSYLPURIN-6-YL)CARBAMOYL]THREONINE-5-MONOPHOSPHATE'>T6A</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g1g OCA], [https://pdbe.org/2g1g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g1g RCSB], [https://www.ebi.ac.uk/pdbsum/2g1g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g1g ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
Modified nucleosides play an important role in structure and function of tRNA. We have determined the solution structure of the anticodon stem-loop (ASL) of initiator tRNA of Schizosaccharomyces pombe. The incorporation of N6-threonylcarbamoyladenosine at the position 3' to the anticodon triplet (t6A37) results in the formation of a U-turn motif and enhances stacking interactions within the loop and stem regions (i.e. between A35 and t6A37) by bulging out U36. This conformation was not observed in a crystal structure of tRNAi including the same modification in its anticodon loop, nor in the solution structure of the unmodified ASL. A t6A modification also occurs in the well studied anti-stem-loop of lys-tRNA(UUU). A comparison of this stem-loop with our structure demonstrates different effects of the modification depending on the loop sequence. | Modified nucleosides play an important role in structure and function of tRNA. We have determined the solution structure of the anticodon stem-loop (ASL) of initiator tRNA of Schizosaccharomyces pombe. The incorporation of N6-threonylcarbamoyladenosine at the position 3' to the anticodon triplet (t6A37) results in the formation of a U-turn motif and enhances stacking interactions within the loop and stem regions (i.e. between A35 and t6A37) by bulging out U36. This conformation was not observed in a crystal structure of tRNAi including the same modification in its anticodon loop, nor in the solution structure of the unmodified ASL. A t6A modification also occurs in the well studied anti-stem-loop of lys-tRNA(UUU). A comparison of this stem-loop with our structure demonstrates different effects of the modification depending on the loop sequence. | ||
| - | + | The naturally occurring N6-threonyl adenine in anticodon loop of Schizosaccharomyces pombe tRNAi causes formation of a unique U-turn motif.,Lescrinier E, Nauwelaerts K, Zanier K, Poesen K, Sattler M, Herdewijn P Nucleic Acids Res. 2006 May 31;34(10):2878-86. Print 2006. PMID:16738127<ref>PMID:16738127</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 2g1g" style="background-color:#fffaf0;"></div> |
| - | [[Category: Lescrinier | + | == References == |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| + | [[Category: Large Structures]] | ||
| + | [[Category: Lescrinier E]] | ||
Current revision
Solution structure of the anticodon loop of S. Pombe tRNAi including the naturally occurring N6-threonyl adenine
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