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| - | [[Image:2g23.gif|left|200px]]<br /><applet load="2g23" size="350" color="white" frame="true" align="right" spinBox="true" | + | #REDIRECT [[3gyr]] This PDB entry is obsolete and replaced by 3gyr |
| - | caption="2g23, resolution 2.30Å" />
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| - | '''The crystal structure of hexameric phenoxazinone synthase'''<br />
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| - | ==Overview==
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| - | The multicopper oxidase phenoxazinone synthase (PHS) catalyzes the penultimate step in the biosynthesis of the antibiotic actinomycin D by Streptomyces antibioticus. PHS exists in two oligomeric forms: a dimeric form and a hexameric form, with older actinomycin-producing cultures containing predominately the hexameric form. The structure of hexameric PHS has been determined using X-ray diffraction to a resolution limit of 2.30 A and is found to contain several unexpected and distinctive features. The structure forms a hexameric ring that is centered on a pseudo 6-fold axis and has an outer diameter of 185 A with a large central cavity that has a diameter of 50 A. This hexameric structure is stabilized by a long loop connecting two domains; bound to this long loop is a fifth copper atom that is present as a type 2 copper. This copper atom is not present in any other multicopper oxidase, and its presence appears to stabilize the hexameric structure.
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| - | ==About this Structure==
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| - | 2G23 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_antibioticus Streptomyces antibioticus] with <scene name='pdbligand=CU:'>CU</scene>, <scene name='pdbligand=C2O:'>C2O</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G23 OCA].
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| - | ==Reference==
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| - | Structure of phenoxazinone synthase from Streptomyces antibioticus reveals a new type 2 copper center., Smith AW, Camara-Artigas A, Wang M, Allen JP, Francisco WA, Biochemistry. 2006 Apr 11;45(14):4378-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16584173 16584173]
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| - | [[Category: Single protein]]
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| - | [[Category: Streptomyces antibioticus]]
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| - | [[Category: Allen, J P.]]
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| - | [[Category: Camara-Artigas, A.]]
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| - | [[Category: Francisco, W A.]]
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| - | [[Category: Smith, A W.]]
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| - | [[Category: Wang, M.]]
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| - | [[Category: C2O]]
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| - | [[Category: CU]]
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| - | [[Category: GOL]]
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| - | [[Category: copper]]
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| - | [[Category: hexamer]]
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| - | [[Category: laccase]]
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| - | [[Category: metalloprotein]]
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| - | [[Category: multicopper oxidase]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:27:26 2008''
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