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4bkk
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The Respiratory Syncytial Virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid.== |
| + | <SX load='4bkk' size='340' side='right' viewer='molstar' caption='[[4bkk]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4bkk]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus_A_strain_Long Human respiratory syncytial virus A strain Long]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BKK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BKK FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bkk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bkk OCA], [https://pdbe.org/4bkk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bkk RCSB], [https://www.ebi.ac.uk/pdbsum/4bkk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bkk ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/NCAP_HRSVA NCAP_HRSVA] Encapsidates the genome, protecting it from nucleases. The nucleocapsid (NC) has a helical structure. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases.<ref>PMID:9299631</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Respiratory syncytial virus (RSV) is an important human pathogen. Its nucleocapsid (NC), which comprises the negative sense RNA viral genome coated by the viral nucleoprotein N, is a critical assembly that serves as template for both mRNA synthesis and genome replication. We have previously described the X-ray structure of an NC-like structure: a decameric ring formed of N-RNA that mimics one turn of the helical NC. In the absence of experimental data we had hypothesized that the NC helix would be right-handed, as the N-N contacts in the ring appeared to more easily adapt to that conformation. We now unambiguously show that the RSV NC is a left-handed helix. We further show that the contacts in the ring can be distorted to maintain key N-N-protein interactions in a left-handed helix, and discuss the implications of the resulting atomic model of the helical NC for viral replication and transcription. | ||
| - | + | The respiratory syncytial virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid.,Bakker SE, Duquerroy S, Galloux M, Loney C, Conner E, Eleouet JF, Rey FA, Bhella D J Gen Virol. 2013 Aug;94(Pt 8):1734-8. doi: 10.1099/vir.0.053025-0. Epub 2013 May, 15. PMID:23677789<ref>PMID:23677789</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4bkk" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Human respiratory syncytial virus A strain Long]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bakker SE]] | ||
| + | [[Category: Bhella D]] | ||
| + | [[Category: Conner E]] | ||
| + | [[Category: Duquerroy S]] | ||
| + | [[Category: Eleouet JF]] | ||
| + | [[Category: Galloux M]] | ||
| + | [[Category: Loney C]] | ||
| + | [[Category: Rey FA]] | ||
Current revision
The Respiratory Syncytial Virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid.
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