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1ump
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| - | [[Image:1ump.gif|left|200px]]<br /> | ||
| - | <applet load="1ump" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1ump, resolution 2.13Å" /> | ||
| - | '''GEOMETRY OF TRITERPENE CONVERSION TO PENTACARBOCYCLIC HOPENE'''<br /> | ||
| - | == | + | ==GEOMETRY OF TRITERPENE CONVERSION TO PENTACARBOCYCLIC HOPENE== |
| - | The membrane protein squalene-hopene cyclase was cocrystallized with | + | <StructureSection load='1ump' size='340' side='right'caption='[[1ump]], [[Resolution|resolution]] 2.13Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1ump]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Alicyclobacillus_acidocaldarius Alicyclobacillus acidocaldarius]. The December 2007 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Oxidosqualene Cyclase'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2007_12 10.2210/rcsb_pdb/mom_2007_12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UMP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UMP FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.13Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=SQA:2-AZASQUALENE'>SQA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ump FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ump OCA], [https://pdbe.org/1ump PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ump RCSB], [https://www.ebi.ac.uk/pdbsum/1ump PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ump ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SQHC_ALIAD SQHC_ALIAD] Catalyzes the cyclization of squalene into hopene. | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/um/1ump_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ump ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The membrane protein squalene-hopene cyclase was cocrystallized with 2-azasqualene and analyzed by X-ray diffraction to 2.13 A resolution. The conformation of this close analog was clearly established, and it agreed with the common textbook presentation. The bound squalene undergoes only small conformational changes during the formation of rings A through D, thus requiring no intermediate. However, ring E formation is hindered by an entropic barrier, which may explain its absence in the steroids. The structure analysis revealed a mobile region between the active center cavity and the membrane, which may melt, opening a passage for squalene and hopene. | ||
| - | + | Conversion of squalene to the pentacarbocyclic hopene.,Reinert DJ, Balliano G, Schulz GE Chem Biol. 2004 Jan;11(1):121-6. PMID:15113001<ref>PMID:15113001</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1ump" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[Squalene-hopene cyclase|Squalene-hopene cyclase]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Alicyclobacillus acidocaldarius]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Oxidosqualene Cyclase]] | ||
| + | [[Category: RCSB PDB Molecule of the Month]] | ||
| + | [[Category: Balliano G]] | ||
| + | [[Category: Reinert DJ]] | ||
| + | [[Category: Schulz GE]] | ||
Current revision
GEOMETRY OF TRITERPENE CONVERSION TO PENTACARBOCYCLIC HOPENE
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