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| - | [[Image:1un7.gif|left|200px]]<br /> | + | #REDIRECT [[2vhl]] This PDB entry is obsolete and replaced by 2vhl |
| - | <applet load="1un7" size="450" color="white" frame="true" align="right" spinBox="true"
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| - | caption="1un7, resolution 2.05Å" />
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| - | '''THE 3-D STRUCTURE OF THE N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE, NAGA, FROM BACILLUS SUBTILIS: A MEMBER OF THE UREASE SUPERFAMILY'''<br />
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| - | ==Overview==
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| - | The enzyme N-acetylglucosamine-6-phosphate deacetylase, NagA, catalyzes, the hydrolysis of the N-acetyl group of GlcNAc-6-P to yield glucosamine, 6-phosphate and acetate, the first committed step in the biosynthetic, pathway to amino-sugar-nucleotides. It is classified into carbohydrate, esterase family CE-9 (see afmb.cnrs-mrs.fr/CAZY/). Here we report the, cloning, expression, and three-dimensional structure (Protein Data Bank, code 1un7) determination by x-ray crystallography of the Bacillus subtilis, NagA at a resolution of 2.0 A. The structure presents two domains, a, (beta/alpha)(8) barrel enclosing the active center and a small beta barrel, domain. The structure is dimeric, and the substrate phosphate coordination, at the active center is provided by an Arg/His pair contributed from ... [[http://ispc.weizmann.ac.il/pmbin/getpm?14557261 (full description)]]
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| - | ==About this Structure==
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| - | 1UN7 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]] with GLP, FE and 2PE as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/N-acetylglucosamine-6-phosphate_deacetylase N-acetylglucosamine-6-phosphate deacetylase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.25 3.5.1.25]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UN7 OCA]].
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| - | ==Reference==
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| - | The three-dimensional structure of the N-acetylglucosamine-6-phosphate deacetylase, NagA, from Bacillus subtilis: a member of the urease superfamily., Vincent F, Yates D, Garman E, Davies GJ, Brannigan JA, J Biol Chem. 2004 Jan 23;279(4):2809-16. Epub 2003 Oct 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14557261 14557261]
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| - | [[Category: Bacillus subtilis]]
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| - | [[Category: N-acetylglucosamine-6-phosphate deacetylase]]
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| - | [[Category: Single protein]]
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| - | [[Category: Brannigan, J.A.]]
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| - | [[Category: Davies, G.J.]]
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| - | [[Category: Garman, E.]]
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| - | [[Category: Vincent, F.]]
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| - | [[Category: Yates, D.]]
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| - | [[Category: 2PE]]
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| - | [[Category: FE]]
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| - | [[Category: GLP]]
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| - | [[Category: bacillus subtilis]]
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| - | [[Category: deacetylase ]]
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| - | [[Category: hydrolase]]
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| - | [[Category: n-acetyleglucosamine-6-phosphate]]
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| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:06:33 2007''
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