3pkq

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{{STRUCTURE_3pkq| PDB=3pkq | SCENE= }}
 
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===Q83D Variant of S. Enterica RmlA with dGTP===
 
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{{ABSTRACT_PUBMED_21317292}}
 
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==About this Structure==
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==Q83D Variant of S. Enterica RmlA with dGTP==
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[[3pkq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PKQ OCA].
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<StructureSection load='3pkq' size='340' side='right'caption='[[3pkq]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3pkq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PKQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DGT:2-DEOXYGUANOSINE-5-TRIPHOSPHATE'>DGT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pkq OCA], [https://pdbe.org/3pkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pkq RCSB], [https://www.ebi.ac.uk/pdbsum/3pkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pkq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RMLA_SALTY RMLA_SALTY] Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis. Is also able to convert non natural substrates such as a wide array of alpha-D-hexopyranosyl, deoxy-alpha-D-glucopyranosyl, aminodeoxy-alpha-D-hexopyranosyl and acetamidodeoxy-alpha-D-hexopyranosyl phosphates to their corresponding dTDP- and UDP-nucleotide sugars.<ref>PMID:8382158</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Directed evolution is a valuable technique to improve enzyme activity in the absence of a priori structural knowledge, which can be typically enhanced via structure-guided strategies. In this study, a combination of both whole-gene error-prone polymerase chain reaction and site-saturation mutagenesis enabled the rapid identification of mutations that improved RmlA activity toward non-native substrates. These mutations have been shown to improve activities over 10-fold for several targeted substrates, including non-native pyrimidine- and purine-based NTPs as well as non-native d- and l-sugars (both alpha- and beta-isomers). This study highlights the first broadly applicable high throughput sugar-1-phosphate nucleotidyltransferase screen and the first proof of concept for the directed evolution of this enzyme class toward the identification of uniquely permissive RmlA variants.
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==Reference==
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Expanding the Nucleotide and Sugar 1-Phosphate Promiscuity of Nucleotidyltransferase RmlA via Directed Evolution.,Moretti R, Chang A, Peltier-Pain P, Bingman CA, Phillips GN Jr, Thorson JS J Biol Chem. 2011 Apr 15;286(15):13235-43. Epub 2011 Feb 11. PMID:21317292<ref>PMID:21317292</ref>
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<ref group="xtra">PMID:021317292</ref><references group="xtra"/><references/>
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[[Category: Glucose-1-phosphate thymidylyltransferase]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
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</div>
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[[Category: Bingman, C A.]]
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<div class="pdbe-citations 3pkq" style="background-color:#fffaf0;"></div>
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[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
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[[Category: Chang, A.]]
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==See Also==
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[[Category: Moretti, R.]]
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*[[Glucose-1-phosphate thymidylyltransferase 3D structures|Glucose-1-phosphate thymidylyltransferase 3D structures]]
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[[Category: Phillips, G N.]]
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== References ==
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[[Category: Thorson, J S.]]
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<references/>
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[[Category: Center for eukaryotic structural genomic]]
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__TOC__
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[[Category: Cesg]]
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</StructureSection>
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[[Category: Directed evolution]]
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[[Category: Large Structures]]
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[[Category: Nucleotidylyltransferase]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Protein structure initiative]]
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[[Category: Bingman CA]]
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[[Category: Psi-2]]
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[[Category: Chang A]]
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[[Category: Structural genomic]]
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[[Category: Moretti R]]
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[[Category: Transferase]]
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[[Category: Phillips Jr GN]]
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[[Category: Thorson JS]]

Current revision

Q83D Variant of S. Enterica RmlA with dGTP

PDB ID 3pkq

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