2gfp

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[[Image:2gfp.gif|left|200px]]<br /><applet load="2gfp" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2gfp, resolution 3.5&Aring;" />
 
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'''Structure of the Multidrug Transporter EmrD from Escherichia coli'''<br />
 
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==Overview==
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==Structure of the Multidrug Transporter EmrD from Escherichia coli==
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EmrD is a multidrug transporter from the Major Facilitator Superfamily that expels amphipathic compounds across the inner membrane of Escherichia coli. Here, we report the x-ray structure of EmrD determined to a resolution of 3.5 angstroms. The structure reveals an interior that is composed mostly of hydrophobic residues, which is consistent with its role transporting amphipathic molecules. Two long loops extend into the inner leaflet side of the cell membrane. This region can serve to recognize and bind substrate directly from the lipid bilayer. We propose that multisubstrate specificity, binding, and transport are facilitated by these loop regions and the internal cavity.
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<StructureSection load='2gfp' size='340' side='right'caption='[[2gfp]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2gfp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The November 2007 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Multidrug Resistance Transporters'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2007_11 10.2210/rcsb_pdb/mom_2007_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GFP FirstGlance]. <br>
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2GFP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GFP OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gfp OCA], [https://pdbe.org/2gfp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gfp RCSB], [https://www.ebi.ac.uk/pdbsum/2gfp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gfp ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structure of the multidrug transporter EmrD from Escherichia coli., Yin Y, He X, Szewczyk P, Nguyen T, Chang G, Science. 2006 May 5;312(5774):741-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16675700 16675700]
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== Function ==
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[https://www.uniprot.org/uniprot/EMRD_ECOLI EMRD_ECOLI] Multidrug resistance pump that participates in a low energy shock adaptative response.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gf/2gfp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gfp ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chang, G.]]
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[[Category: Multidrug Resistance Transporters]]
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[[Category: He, X.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Nguyen, T.]]
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[[Category: Chang G]]
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[[Category: Szewczyk, P.]]
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[[Category: He X]]
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[[Category: Yin, Y.]]
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[[Category: Nguyen T]]
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[[Category: membrane protein; multidrug transporter]]
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[[Category: Szewczyk P]]
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[[Category: Yin Y]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:31:15 2008''
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Current revision

Structure of the Multidrug Transporter EmrD from Escherichia coli

PDB ID 2gfp

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