This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2gns

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:49, 25 October 2023) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2gns.gif|left|200px]]<br /><applet load="2gns" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2gns, resolution 2.30&Aring;" />
 
-
'''Design of specific peptide inhibitors of phospholipase A2: Crystal structure of the complex formed between a group II phospholipase A2 and a designed pentapeptide Ala- Leu- Val- Tyr- Lys at 2.3 A resolution'''<br />
 
-
==About this Structure==
+
==Design of specific peptide inhibitors of phospholipase A2: Crystal structure of the complex formed between a group II phospholipase A2 and a designed pentapeptide Ala- Leu- Val- Tyr- Lys at 2.3 A resolution==
-
2GNS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Daboia_russellii_pulchella Daboia russellii pulchella] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GNS OCA].
+
<StructureSection load='2gns' size='340' side='right'caption='[[2gns]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-
[[Category: Daboia russellii pulchella]]
+
== Structural highlights ==
-
[[Category: Phospholipase A(2)]]
+
<table><tr><td colspan='2'>[[2gns]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Daboia_russelii_pulchella Daboia russelii pulchella]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GNS FirstGlance]. <br>
-
[[Category: Single protein]]
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-
[[Category: Dey, S.]]
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
[[Category: Sharma, S.]]
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gns OCA], [https://pdbe.org/2gns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gns RCSB], [https://www.ebi.ac.uk/pdbsum/2gns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gns ProSAT]</span></td></tr>
-
[[Category: Singh, N.]]
+
</table>
-
[[Category: Singh, T P.]]
+
== Function ==
-
[[Category: Somvanshi, R K.]]
+
[https://www.uniprot.org/uniprot/PA2B8_DABRR PA2B8_DABRR] Snake venom phospholipase A2 (PLA2) that shows weak neurotoxicity and medium anticoagulant effects by binding to factor Xa (F10) and inhibiting the prothrombinase activity (IC(50) is 130 nM) (PubMed:18062812). It also damages vital organs such as lung, liver and kidney, displays edema-inducing activities when injected into the foot pads of mice and induces necrosis of muscle cells when injected into the thigh muscle. Has a low enzymatic activity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.<ref>PMID:18062812</ref> <ref>PMID:2115497</ref> <ref>PMID:8835338</ref>
-
[[Category: SO4]]
+
== Evolutionary Conservation ==
-
[[Category: binding constant]]
+
[[Image:Consurf_key_small.gif|200px|right]]
-
[[Category: peptide inhibitor]]
+
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gn/2gns_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gns ConSurf].
 +
<div style="clear:both"></div>
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:33:33 2008''
+
==See Also==
 +
*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Daboia russelii pulchella]]
 +
[[Category: Large Structures]]
 +
[[Category: Dey S]]
 +
[[Category: Sharma S]]
 +
[[Category: Singh N]]
 +
[[Category: Singh TP]]
 +
[[Category: Somvanshi RK]]

Current revision

Design of specific peptide inhibitors of phospholipase A2: Crystal structure of the complex formed between a group II phospholipase A2 and a designed pentapeptide Ala- Leu- Val- Tyr- Lys at 2.3 A resolution

PDB ID 2gns

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gns"
Personal tools