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| - | [[Image:2goz.gif|left|200px]]<br /><applet load="2goz" size="350" color="white" frame="true" align="right" spinBox="true" | + | #REDIRECT [[3zd5]] This PDB entry is obsolete and replaced by 3zd5 |
| - | caption="2goz, resolution 2.20Å" />
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| - | '''The 2.2 A structure of a full-length catalytically active hammerhead ribozyme'''<br />
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| - | ==Overview==
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| - | Minimal hammerhead ribozymes have been characterized extensively by static and time-resolved crystallography as well as numerous biochemical analyses, leading to mutually contradictory mechanistic explanations for catalysis. We present the 2.2 A resolution crystal structure of a full-length Schistosoma mansoni hammerhead ribozyme that permits us to explain the structural basis for its 1000-fold catalytic enhancement. The full-length hammerhead structure reveals how tertiary interactions occurring remotely from the active site prime this ribozyme for catalysis. G-12 and G-8 are positioned consistent with their previously suggested roles in acid-base catalysis, the nucleophile is aligned with a scissile phosphate positioned proximal to the A-9 phosphate, and previously unexplained roles of other conserved nucleotides become apparent within the context of a distinctly new fold that nonetheless accommodates the previous structural studies. These interactions permit us to explain the previously irreconcilable sets of experimental results in a unified, consistent, and unambiguous manner.
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| - | ==About this Structure==
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| - | 2GOZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GOZ OCA].
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| - | ==Reference==
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| - | Tertiary contacts distant from the active site prime a ribozyme for catalysis., Martick M, Scott WG, Cell. 2006 Jul 28;126(2):309-20. Epub 2006 Jul 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16859740 16859740]
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| - | [[Category: Protein complex]]
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| - | [[Category: Martick, M.]]
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| - | [[Category: Scott, W G.]]
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| - | [[Category: a-form helix]]
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| - | [[Category: catalytic rna]]
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| - | [[Category: hammerhead ribozyme]]
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| - | [[Category: hammerhead rna]]
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| - | [[Category: in-line attack]]
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| - | [[Category: ribozyme]]
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| - | [[Category: stem-loop interaction]]
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| - | [[Category: uridine turn]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:33:50 2008''
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