3rfv
From Proteopedia
(Difference between revisions)
| (3 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | {{STRUCTURE_3rfv| PDB=3rfv | SCENE= }} | ||
| - | ===Crystal structure of Uronate dehydrogenase from Agrobacterium tumefaciens complexed with NADH and product=== | ||
| - | {{ABSTRACT_PUBMED_21676870}} | ||
| - | == | + | ==Crystal structure of Uronate dehydrogenase from Agrobacterium tumefaciens complexed with NADH and product== |
| - | [[3rfv]] is a 3 chain structure with sequence from [ | + | <StructureSection load='3rfv' size='340' side='right'caption='[[3rfv]], [[Resolution|resolution]] 2.10Å' scene=''> |
| - | + | == Structural highlights == | |
| - | == | + | <table><tr><td colspan='2'>[[3rfv]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_fabrum_str._C58 Agrobacterium fabrum str. C58]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RFV FirstGlance]. <br> |
| - | < | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | [[ | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=15L:GALACTARO-1,5-LACTONE'>15L</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | [ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rfv OCA], [https://pdbe.org/3rfv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rfv RCSB], [https://www.ebi.ac.uk/pdbsum/3rfv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rfv ProSAT]</span></td></tr> |
| - | + | </table> | |
| - | [ | + | == Function == |
| - | [[Category: | + | [https://www.uniprot.org/uniprot/URODH_AGRFC URODH_AGRFC] Catalyzes the oxidation of D-galacturonate and D-glucuronate to galactarate and D-glucarate, respectively. In fact, in water solution the substrate D-galacturonate is predominantly in pyranosic form whose beta anomer is converted by the enzyme to D-galactaro-1,5-lactone; in solution, this reaction product rearranges to the more stable D-galactaro-1,4-lactone. Makes part of the oxidative degradation pathway of D-galacturonate, which allows A.tumefaciens to utilize D-galacturonate as a sole carbon source. Cannot use NADP(+) instead of NAD(+) as cosubstrate. Is not active on D-galactose, D-glucose, D-galactonate and D-gluconate.<ref>PMID:19060141</ref> <ref>PMID:19921179</ref> <ref>PMID:21676870</ref> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Agrobacterium fabrum str. C58]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Parkkinen T]] | ||
| + | [[Category: Rouvinen J]] | ||
Current revision
Crystal structure of Uronate dehydrogenase from Agrobacterium tumefaciens complexed with NADH and product
| |||||||||||
