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3rcb

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{{STRUCTURE_3rcb| PDB=3rcb | SCENE= }}
 
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===Crystal structure of the K102E mutant of KijD10, a 3-ketoreductase from Actinomadura kijaniata in complex with TDP-benzene and NADP===
 
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{{ABSTRACT_PUBMED_21598943}}
 
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==About this Structure==
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==Crystal structure of the K102E mutant of KijD10, a 3-ketoreductase from Actinomadura kijaniata in complex with TDP-benzene and NADP==
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[[3rcb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Actinomadura_kijaniata Actinomadura kijaniata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCB OCA].
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<StructureSection load='3rcb' size='340' side='right'caption='[[3rcb]], [[Resolution|resolution]] 2.49&Aring;' scene=''>
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== Structural highlights ==
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==Reference==
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<table><tr><td colspan='2'>[[3rcb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinomadura_kijaniata Actinomadura kijaniata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RCB FirstGlance]. <br>
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<ref group="xtra">PMID:021598943</ref><references group="xtra"/><references/>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.49&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TLO:5-O-[(S)-HYDROXY{[(S)-HYDROXY(PHENOXY)PHOSPHORYL]OXY}PHOSPHORYL]THYMIDINE'>TLO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rcb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rcb OCA], [https://pdbe.org/3rcb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rcb RCSB], [https://www.ebi.ac.uk/pdbsum/3rcb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rcb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KIJDR_ACTKI KIJDR_ACTKI] Involved in the biosynthesis of L-digitoxose, an unusual dideoxysugar attached to various pharmacologically active natural products, including the antitumor antibiotic tetrocarcin A, and the antibiotics kijanimicin and jadomycin B. Catalyzes the reduction of the C-3 keto moiety of dTDP-3,4-diketo-2,6-dideoxy-alpha-D-glucose to yield dTDP-4-keto-2,6-dideoxy-alpha-D-glucose. Also able to reduce dTDP-3-keto-6-deoxy-D-galactose and dTDP-3-keto-6-deoxy-D-glucose to yield dTDP-fucose and dTDP-quinovose, respectively.<ref>PMID:21598943</ref>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Actinomadura kijaniata]]
[[Category: Actinomadura kijaniata]]
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[[Category: Holden, H M.]]
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[[Category: Large Structures]]
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[[Category: Kubiak, R L.]]
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[[Category: Holden HM]]
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[[Category: Ketoreductase]]
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[[Category: Kubiak RL]]
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[[Category: Nadp binding]]
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[[Category: Sugar binding protein]]
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[[Category: Sugar biosynthesis]]
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[[Category: Tdp binding]]
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Current revision

Crystal structure of the K102E mutant of KijD10, a 3-ketoreductase from Actinomadura kijaniata in complex with TDP-benzene and NADP

PDB ID 3rcb

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