3k3r

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{{STRUCTURE_3k3r| PDB=3k3r | SCENE= }}
 
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===Unrefined crystal structure of a LexA-DNA complex===
 
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{{ABSTRACT_PUBMED_20703307}}
 
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==Function==
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==Unrefined crystal structure of a LexA-DNA complex==
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[[http://www.uniprot.org/uniprot/LEXA_ECOLI LEXA_ECOLI]] Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.<ref>PMID:7027255</ref> <ref>PMID:7027256</ref>
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<StructureSection load='3k3r' size='340' side='right'caption='[[3k3r]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[3k3r]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K3R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K3R FirstGlance]. <br>
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[[3k3r]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K3R OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k3r OCA], [https://pdbe.org/3k3r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k3r RCSB], [https://www.ebi.ac.uk/pdbsum/3k3r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k3r ProSAT]</span></td></tr>
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==Reference==
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</table>
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<ref group="xtra">PMID:020703307</ref><references group="xtra"/><references/>
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== Function ==
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[[Category: Escherichia coli k-12]]
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[https://www.uniprot.org/uniprot/LEXA_ECOLI LEXA_ECOLI] Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.<ref>PMID:7027255</ref> <ref>PMID:7027256</ref>
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[[Category: Repressor lexA]]
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== Evolutionary Conservation ==
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[[Category: Pigli, Y Z.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Rice, P A.]]
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Check<jmol>
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[[Category: Zhang, A P.P.]]
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<jmolCheckbox>
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[[Category: Autocatalytic cleavage]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k3/3k3r_consurf.spt"</scriptWhenChecked>
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[[Category: Dna damage]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Dna repair]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Dna replication]]
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</jmolCheckbox>
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[[Category: Dna-binding]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3k3r ConSurf].
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[[Category: Double helix]]
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<div style="clear:both"></div>
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[[Category: Hydrolase]]
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== References ==
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[[Category: Hydrolase-dna complex]]
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<references/>
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[[Category: Lexa]]
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__TOC__
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[[Category: Protein-dna complex]]
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</StructureSection>
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[[Category: Repressor]]
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[[Category: Escherichia coli K-12]]
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[[Category: Sos response]]
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[[Category: Large Structures]]
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[[Category: Sos system]]
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[[Category: Pigli YZ]]
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[[Category: Transcription]]
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[[Category: Rice PA]]
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[[Category: Transcription regulation]]
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[[Category: Zhang APP]]
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[[Category: Winged helix-turn-helix]]
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Current revision

Unrefined crystal structure of a LexA-DNA complex

PDB ID 3k3r

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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