2gzf

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[[Image:2gzf.jpg|left|200px]]<br /><applet load="2gzf" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2gzf, resolution 1.750&Aring;" />
 
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'''Crystal structure of the E9 DNase domain with a mutant immunity protein IM9 (Y54F)'''<br />
 
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==Overview==
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==Crystal structure of the E9 DNase domain with a mutant immunity protein IM9 (Y54F)==
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The crystal structure of the cytotoxic endonuclease domain from the bacterial toxin colicin E9 in complex with its cognate immunity protein Im9 reveals that the inhibitor does not bind at the active site, the core of which comprises the HNH motif found in intron-encoded homing endonucleases, but rather at an adjacent position leaving the active site exposed yet unable to bind DNA because of steric and electrostatic clashes with incoming substrate. Although its mode of action is unorthodox, Im9 is a remarkably effective inhibitor since it folds within milliseconds and then associates with its target endonuclease at the rate of diffusion to form an inactive complex with sub-femtomolar binding affinity. This hyperefficient mechanism of inhibition could be well suited to other toxic enzyme systems, particularly where the substrate is a polymer extending beyond the boundaries of the active site.
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<StructureSection load='2gzf' size='340' side='right'caption='[[2gzf]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2gzf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GZF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GZF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gzf OCA], [https://pdbe.org/2gzf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gzf RCSB], [https://www.ebi.ac.uk/pdbsum/2gzf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gzf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IMM9_ECOLX IMM9_ECOLX] This protein is able to protect a cell, which harbors the plasmid ColE9 encoding colicin E9, against colicin E9, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gz/2gzf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gzf ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2GZF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Deoxyribonuclease_I Deoxyribonuclease I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.1 3.1.21.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GZF OCA].
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*[[Colicin 3D structures|Colicin 3D structures]]
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*[[Colicin immunity protein 3D structures|Colicin immunity protein 3D structures]]
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==Reference==
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__TOC__
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Structural and mechanistic basis of immunity toward endonuclease colicins., Kleanthous C, Kuhlmann UC, Pommer AJ, Ferguson N, Radford SE, Moore GR, James R, Hemmings AM, Nat Struct Biol. 1999 Mar;6(3):243-52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10074943 10074943]
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</StructureSection>
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[[Category: Deoxyribonuclease I]]
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[[Category: Escherichia coli K-12]]
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Hemmings AM]]
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[[Category: Hemmings, A M.]]
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[[Category: Kleanthous C]]
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[[Category: Kleanthous, C.]]
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[[Category: Kolade OO]]
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[[Category: Kolade, O O.]]
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[[Category: Kuhlmann UC]]
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[[Category: Kuhlmann, U C.]]
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[[Category: Santi PS]]
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[[Category: Santi, P S.]]
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[[Category: PO4]]
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[[Category: ZN]]
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[[Category: 4-helix bundle]]
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[[Category: dnase domain]]
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[[Category: hnh-motif]]
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[[Category: protein-protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:36:41 2008''
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Current revision

Crystal structure of the E9 DNase domain with a mutant immunity protein IM9 (Y54F)

PDB ID 2gzf

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