2h1m

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[[Image:2h1m.gif|left|200px]]<br /><applet load="2h1m" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2h1m, resolution 2.90&Aring;" />
 
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'''Synthesis, Oxidation Behavior, Crystallization and Structure of 2'-Methylseleno Guanosine Containing RNAs'''<br />
 
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==Overview==
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==Synthesis, Oxidation Behavior, Crystallization and Structure of 2'-Methylseleno Guanosine Containing RNAs==
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We have recently introduced a basic concept for the combined chemical and enzymatic preparation of site-specifically modified 2'-methylseleno RNAs which represent useful derivatives for phasing of X-ray crystallographic data during their three-dimensional structure determination. Here, we introduce the first synthesis of an appropriate guanosine phosphoramidite, which complements the thus far established set of 2'-methylseleno-modified uridine, cytidine, and adenosine building blocks for solid-phase synthesis. The novel building block was readily incorporated into RNA. Importantly, it was the 2'-methylseleno-guanosine-labeled RNA that allowed us to reveal the reversible oxidation/reduction behavior of the Se moiety and thus it represents a valuable contribution to the understanding of the action of threo-1,4-dimercapto-2,3-butanediol (DTT) required during solid-phase synthesis, deprotection, and crystallization of selenium-containing RNA. In addition, we investigated 2'-methylseleno RNA with respect to crystallization properties. Our studies revealed that the Se modification significantly increases the range of conditions leading to crystal growth. Moreover, we determined the crystal structures of model RNA helices and showed that the Se modification can affect crystal packing interactions, thus potentially expanding the possibilities for obtaining the best crystal form.
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<StructureSection load='2h1m' size='340' side='right'caption='[[2h1m]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2h1m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H1M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H1M FirstGlance]. <br>
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2H1M is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H1M OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XUG:2-SE-METHYL-2-SELENOGUANOSINE+5-(DIHYDROGEN+PHOSPHATE)'>XUG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h1m OCA], [https://pdbe.org/2h1m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h1m RCSB], [https://www.ebi.ac.uk/pdbsum/2h1m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h1m ProSAT]</span></td></tr>
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Synthesis, oxidation behavior, crystallization and structure of 2'-methylseleno guanosine containing RNAs., Moroder H, Kreutz C, Lang K, Serganov A, Micura R, J Am Chem Soc. 2006 Aug 2;128(30):9909-18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16866550 16866550]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Serganov, A A.]]
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</StructureSection>
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[[Category: SO4]]
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[[Category: Large Structures]]
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[[Category: a-rna structure]]
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[[Category: Serganov AA]]
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[[Category: mismatch a rna]]
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[[Category: ribonucleic acid]]
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[[Category: se 2'-methylse]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:37:16 2008''
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Synthesis, Oxidation Behavior, Crystallization and Structure of 2'-Methylseleno Guanosine Containing RNAs

PDB ID 2h1m

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