4kqe

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(New page: '''Unreleased structure''' The entry 4kqe is ON HOLD until Paper Publication Authors: Qin, X., Hao, Z., Tian, Q., Zhang, Z., Zhou, C., Xie, W. Description: Complex structure reveals mu...)
Current revision (14:31, 8 November 2023) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 4kqe is ON HOLD until Paper Publication
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==The mutant structure of the human glycyl-tRNA synthetase E71G==
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<StructureSection load='4kqe' size='340' side='right'caption='[[4kqe]], [[Resolution|resolution]] 2.74&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4kqe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KQE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KQE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.739&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kqe OCA], [https://pdbe.org/4kqe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kqe RCSB], [https://www.ebi.ac.uk/pdbsum/4kqe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kqe ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/GARS_HUMAN GARS_HUMAN] Autosomal dominant Charcot-Marie-Tooth disease type 2D;Distal hereditary motor neuropathy type 5. The disease is caused by variants affecting the gene represented in this entry. The disease is caused by variants affecting the gene represented in this entry. The disease is caused by variants affecting the gene represented in this entry.
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== Function ==
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[https://www.uniprot.org/uniprot/GARS_HUMAN GARS_HUMAN] Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PubMed:17544401, PubMed:28675565, PubMed:24898252). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis (PubMed:19710017).<ref>PMID:17544401</ref> <ref>PMID:19710017</ref> <ref>PMID:24898252</ref> <ref>PMID:28675565</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glycyl-tRNA synthetase (GlyRS) is the enzyme that covalently links glycine to cognate tRNA for translation. It is of great research interest because of its nonconserved quaternary structures, unique species-specific aminoacylation properties, and noncanonical functions in neurological diseases, but none of these is fully understood. We report two crystal structures of human GlyRS variants, in the free form and in complex with tRNA(Gly) respectively, and reveal new aspects of the glycylation mechanism. We discover that insertion 3 differs considerably in conformation in catalysis and that it acts like a "switch" and fully opens to allow tRNA to bind in a cross-subunit fashion. The flexibility of the protein is supported by molecular dynamics simulation, as well as enzymatic activity assays. The biophysical and biochemical studies suggest that human GlyRS may utilize its flexibility for both the traditional function (regulate tRNA binding) and alternative functions (roles in diseases).
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Authors: Qin, X., Hao, Z., Tian, Q., Zhang, Z., Zhou, C., Xie, W.
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Large Conformational Changes of Insertion 3 in Human Glycyl-tRNA Synthetase (hGlyRS) during Catalysis.,Deng X, Qin X, Chen L, Jia Q, Zhang Y, Zhang Z, Lei D, Ren G, Zhou Z, Wang Z, Li Q, Xie W J Biol Chem. 2016 Mar 11;291(11):5740-52. doi: 10.1074/jbc.M115.679126. Epub 2016, Jan 21. PMID:26797133<ref>PMID:26797133</ref>
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Description: Complex structure reveals multiple conformations of glycyl-tRNA synthetase in glycylation
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4kqe" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Hao Z]]
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[[Category: Qin X]]
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[[Category: Tian Q]]
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[[Category: Xie W]]
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[[Category: Zhang Z]]
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[[Category: Zhou C]]

Current revision

The mutant structure of the human glycyl-tRNA synthetase E71G

PDB ID 4kqe

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