4l3t
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4l3t is ON HOLD Authors: King, J.V., Liang, W.G., Tang, W.J. Description: Crystal Structure of Substrate-free Human Presequence Protease) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of Substrate-free Human Presequence Protease== | |
| + | <StructureSection load='4l3t' size='340' side='right'caption='[[4l3t]], [[Resolution|resolution]] 2.03Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4l3t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L3T FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CAS:S-(DIMETHYLARSENIC)CYSTEINE'>CAS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene>, <scene name='pdbligand=MLZ:N-METHYL-LYSINE'>MLZ</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l3t OCA], [https://pdbe.org/4l3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l3t RCSB], [https://www.ebi.ac.uk/pdbsum/4l3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l3t ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PREP_HUMAN PREP_HUMAN] ATP-independent protease that degrades mitochondrial transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference.<ref>PMID:16849325</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Human presequence protease (hPreP) is an M16 metalloprotease localized in mitochondria. There, hPreP facilitates proteostasis by utilizing an approximately 13,300-A3 catalytic chamber to degrade a diverse array of potentially toxic peptides, including mitochondrial presequences and beta-amyloid (Abeta), the latter of which contributes to Alzheimer disease pathogenesis. Here, we report crystal structures for hPreP alone and in complex with Abeta, which show that hPreP uses size exclusion and charge complementation for substrate recognition. These structures also reveal hPreP-specific features that permit a diverse array of peptides, with distinct distributions of charged and hydrophobic residues, to be specifically captured, cleaved, and have their amyloidogenic features destroyed. SAXS analysis demonstrates that hPreP in solution exists in dynamic equilibrium between closed and open states, with the former being preferred. Furthermore, Abeta binding induces the closed state and hPreP dimerization. Together, these data reveal the molecular basis for flexible yet specific substrate recognition and degradation by hPreP. | ||
| - | + | Molecular Basis of Substrate Recognition and Degradation by Human Presequence Protease.,King JV, Liang WG, Scherpelz KP, Schilling AB, Meredith SC, Tang WJ Structure. 2014 Jun 11. pii: S0969-2126(14)00143-9. doi:, 10.1016/j.str.2014.05.003. PMID:24931469<ref>PMID:24931469</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4l3t" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: King JV]] | ||
| + | [[Category: Liang WG]] | ||
| + | [[Category: Tang WJ]] | ||
Current revision
Crystal Structure of Substrate-free Human Presequence Protease
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