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| - | [[Image:2hcy.gif|left|200px]]<br /><applet load="2hcy" size="350" color="white" frame="true" align="right" spinBox="true" | + | #REDIRECT [[4w6z]] This PDB entry is obsolete and replaced by 4w6z |
| - | caption="2hcy, resolution 2.44Å" />
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| - | '''Yeast Alcohol Dehydrogenase I, Saccharomyces cerevisiae fermentative enzyme'''<br />
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| - | ==Overview==
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| - | The cytoplasmic yeast alcohol dehydrogenase I crystallized at 5 degrees C as hexagonal plates or short columns in the presence of NAD+ and 2,2,2-trifluoroethanol, in sodium N-tris(hydroxymethyl)methyl-3-aminopropanesulfonate buffer at pH 8.2 to 8.6, using polyethylene glycol 4000 as precipitant. X-ray diffraction data to 3.2 A resolution show that the crystals are hexagonal in space group P622 with unit cell dimensions a = b = 147.9 A, c = 69.1 A. There is one subunit of the tetrameric enzyme per asymmetric unit, giving a packing density of 2.9 A3/Da.
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| - | ==About this Structure==
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| - | 2HCY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=8ID:'>8ID</scene> and <scene name='pdbligand=ETF:'>ETF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HCY OCA].
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| - | ==Reference==
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| - | Crystallization and preliminary crystallographic studies of Saccharomyces cerevisiae alcohol dehydrogenase I., Ramaswamy S, Kratzer DA, Hershey AD, Rogers PH, Arnone A, Eklund H, Plapp BV, J Mol Biol. 1994 Jan 14;235(2):777-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8289298 8289298]
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| - | [[Category: Alcohol dehydrogenase]]
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| - | [[Category: Saccharomyces cerevisiae]]
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| - | [[Category: Single protein]]
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| - | [[Category: Plapp, B V.]]
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| - | [[Category: Ramaswamy, S.]]
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| - | [[Category: Savarimuthu, B R.]]
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| - | [[Category: 8ID]]
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| - | [[Category: ETF]]
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| - | [[Category: ZN]]
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| - | [[Category: intramolecular disulfide bonds]]
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| - | [[Category: oxidoreductase]]
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| - | [[Category: tetramer of asymmetric dimers]]
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| - | [[Category: zinc coordination]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:40:34 2008''
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